Additional file 7.
Movie S7. The trafficking of protein bodies is dependent on intact microfilaments. A 25-μM solution of latrunculin B, a drug responsible for inducing disintegration of the actin cytoskeleton, was infiltrated into the abaxial surface of Nicotiana benthamiana leaves that were transiently expressing pPGEK. After 1 h of treatment, the infiltrated area was observed by time-lapse imaging via confocal microscopy. Depolymerization of the cytoskeleton prevented all translational movement of the induced protein bodies (PBs), demonstrating that intact microfilaments are necessary for normal PB trafficking. Fifty image frames were taken over the course of 2 min 37 s.
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Conley et al. BMC Biology 2009 7:48 doi:10.1186/1741-7007-7-48