Additional file 4.
Movie S4. Movement of the protein bodies within Nicotiana benthamiana leaf epidermal cells. Time-lapse confocal imaging of cells expressing endoplasmic reticulum- (ER-)targeted green fluorescent protein-elastin-like polypeptide (pPGEK) was performed to demonstrate the mobility of the novel protein bodies (PBs). A variety of patterns of movement were observed for the PBs. The PBs did not generally move with a constant velocity; rather, they sporadically moved about in a stop-and-go, saltatory fashion. Trafficking of the PBs appeared to occur along the underlying cortical ER network in the leaf cells. Sixty image frames were taken over the course of 2 min 58 s.
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Conley et al. BMC Biology 2009 7:48 doi:10.1186/1741-7007-7-48