Schematic representation of the genetic constructs used for Agrobacterium-mediated transient expression in Nicotiana benthamiana leaves. All transgene expression fragments were placed under the control of the cauliflower mosaic virus 35S promoter, a tCUP 5'-untranslated region and the nopaline synthase terminator. RuB-TP, transit peptide from the tobacco small subunit RuBisCo gene; Pr1b-SP, tobacco secretory signal peptide; N-glyco, N-glycosylation motif (GELVSNGTVT); BiP, tobacco immunoglobulin heavy chain binding protein; GFP, green fluorescent protein; YFP, yellow fluorescent protein; CFP, cyan fluorescent protein; ELP, elastin-like polypeptide tag (28×VPGVG); KDEL, endoplasmic reticulum retention signal.
Conley et al. BMC Biology 2009 7:48 doi:10.1186/1741-7007-7-48