Figure 1.

TBP2 is actively down regulated upon meiotic maturation. (A) Western blot analysis of TBP expression in oocytes, eggs and late blastula (stage 9) embryos with four different antibodies; 58C9, SL27, SL30 and SL33. Asterisk indicates non-specific bands. (B) Western blot analysis of TBP2 expression in oocyte germinal vesicles and embryonic nuclei with a TBP2-specific monoclonal antibody 3E6. (C) Stage VI Xenopus oocytes were incubated in MBSH buffer containing 1 μg/ml progesterone. Batches of 15 oocytes were collected at indicated time intervals and analyzed by western blotting. (D) Stage VI Xenopus oocytes injected with TBP and HA-TBP2 mRNA were incubated in MBSH buffer containing 1 μg/ml progesterone. Batches of 15 oocytes were collected at indicated time intervals and analyzed by western blotting. (E) Oocytes expressing exogenous TBP were treated with either progesterone (1 μg/ml) or cycloheximide (15 μg/ml). Batches of 15 oocytes were collected 4 hr and 16 hr after the treatment and analyzed by western blotting. Asterisk indicates non-specific bands.

Akhtar and Veenstra BMC Biology 2009 7:45   doi:10.1186/1741-7007-7-45
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