tTA and hidAla5 expression depending on different integration sites. The expression of tTA and hidAla5 is shown by WMISH performed on embryos from a 0–48 h egg collection of medfly combinations (comb.) carrying both driver (D) sryα 2-tTA and effector (E) TREhs43-hidAla5 in heterozygous conditions. The developmental stages of embryogenesis are as indicated in Figure 2. The flies were reared on Tc-free adult food. 100% lethality in lab tests is indicated with + and the stage of complete lethality is indicated in brackets.
Schetelig et al. BMC Biology 2009 7:4 doi:10.1186/1741-7007-7-4