Analysis of candidate Aplysia californica chemosensory receptors subfamily a. (a) Conservation of predicted amino acid sequences for 10 full-length genes is displayed as a consensus strength as color-coded histogram. In this representation, the relative frequency with which an amino acid appears at a given position is reflected by the color, as depicted by the scale bar. The seven transmembrane domains (based on the HMMTOP version 2.0 program) are indicated by a solid bar above the sequence. (b) Schematic representation of the genome organization of clustered genes, partial AcCR14a and full-length AcCR27a (genomic contig AASC01105652), including predicted start (ATG) and stop codons (TAG) and intron/exon structure of AcCR27a leading to the mature multi-transmembrane protein. Met, methionine. (c) Comparative amino acid alignment of AcCR14a and AcCR27a. Identical amino acids are highlighted in black. Putative intracellular (IC), extracellular (EC), N-terminus and C-terminus domains are shown. Arrow indicates the intron/exon boundary; asterisks show potential N-linked glycosylation sites; black circles represent highly conserved cysteines.
Cummins et al. BMC Biology 2009 7:28 doi:10.1186/1741-7007-7-28