Single molecule resolution of the antimicrobial action of quantum dot-labeled sushi peptide on live bacteria

doi:10.1186/1741-7007-7-22

BMC Biology

Volume 7

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Research article

Single molecule resolution of the antimicrobial action of quantum dot-labeled sushi peptide on live bacteria

Sebastian Leptihn¹^* , Jia Yi Har¹^* , Jianzhu Chen¹^,2 , Bow Ho³ , Thorsten Wohland¹^,4^* and Jeak Ling Ding¹^,5^*

¹Singapore-MIT Alliance, 117576, Singapore

²Koch Institute for Integrative Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA

³Department of Microbiology, Yong Loo Lin School of Medicine, National University of Singapore, 117597, Singapore

⁴Department of Chemistry, National University of Singapore, 117543, Singapore

⁵Department of Biological Sciences, National University of Singapore, 117543, Singapore

author email corresponding author email* Contributed equally

BMC Biology 2009, 7:22doi:10.1186/1741-7007-7-22


Published:	11 May 2009

Additional files

Additional file 1:

Biochemical testing of S1 and the control peptide negS1. (A) Fluorescence emission spectra of negS1 in buffer (dotted line) or in POPS (dash-dot line). For comparison S1 in buffer (solid line) and in POPS is shown (dashed line) (B) Recombinant Factor C activation assay using negS1 (open triangle) and S1 (solid circle). The activation of Factor C without lipopolysaccharide binding peptide is displayed as a solid square. The samples contained 125 nM of the peptides or no peptide. (C) Fluorescence spectroscopy of Qdot655 labeled Escherichia coli. After subtraction of the E. coli auto-fluorescence the biotin-Qdot showed a signal close to zero (dotted line). In contrast, there was a clear fluorescence peak for S1-Qdot labeled cells (solid line), negS1-Qdot show only minor fluorescence (dashed line). Settings: Excitation 488 (15 nm), Emission 600–750 (15 nm).

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Additional file 2:

Table S1. Quantification of S1-nanoparticles counted on 20 Escherichia coli cells (total particle count 254) in transmission electron microscopy micrographs.

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