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Open Access Research article

F-actin-based extensions of the head cyst cell adhere to the maturing spermatids to maintain them in a tight bundle and prevent their premature release in Drosophila testis

Bela S Desai, Seema Shirolikar and Krishanu Ray*

Author Affiliations

Department of Biological Sciences, Tata Institute of Fundamental Research, Homi Bhabha Road, Mumbai 400005, India

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BMC Biology 2009, 7:19  doi:10.1186/1741-7007-7-19


The electronic version of this article is the complete one and can be found online at: http://www.biomedcentral.com/1741-7007/7/19


Received:3 April 2009
Accepted:5 May 2009
Published:5 May 2009

© 2009 Desai et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

In Drosophila, all the 64 clonally derived spermatocytes differentiate in syncytium inside two somatic-origin cyst cells. They elongate to form slender spermatids, which are individualized and then released into the seminal vesicle. During individualization, differentiating spermatids are organized in a tight bundle inside the cyst, which is expected to play an important role in sperm selection. However, actual significance of this process and its underlying mechanism are unclear.

Results

We show that dynamic F-actin-based processes extend from the head cyst cell at the start of individualization, filling the interstitial space at the rostral ends of the maturing spermatid bundle. In addition to actin, these structures contained lamin, beta-catenin, dynamin, myosin VI and several other filopodial components. Further, pharmacological and genetic analyses showed that cytoskeletal stability and dynamin function are essential for their maintenance. Disruption of these F-actin based processes was associated with spermatid bundle disassembly and premature sperm release inside the testis.

Conclusion

Altogether, our data suggests that the head cyst cell adheres to the maturing spermatid heads through F-actin-based extensions, thus maintaining them in a tight bundle. This is likely to regulate mature sperm release into the seminal vesicle. Overall, this process bears resemblance to mammalian spermiation.

Background

Spermiogenesis offers a good model for investigating the molecular basis of large-scale cellular morphogenesis and movement. In Drosophila , 64 haploid sperm develop from a single gonial precursor through several well-defined morphogenetic steps [1,2]. This entire process happens in three distinct developmental stages: (1) the formation of 64 spermatids from a gonial precursor; (2) the elongation of spermatids from nearly spherical to around 1.8 mm long cells; and (3) the individualization of the elongated spermatids into mature sperm, which then enter the seminal vesicle (SV) [2]. Altogether, it involves large-scale changes in cell shape and internal reorganization [3,4]. Every spermatogonial cell is encapsulated by two somatic-origin cyst cells within the testicular lumen as they form at the testis apex. Subsequent developments occur within this cyst capsule. At the end of the process, the individualized sperm coil up inside the cyst capsule at the base of the testis before entering the SV [4]. Studies in Drosophila indicated that defective sperm fail to enter the SV as they fail to remain attached to the head cyst cell during the coiling process [1]. Thus, the dynamics of sperm head attachment to the cyst cell are likely to play a major role in this quality control exercise.

Spermatids grow in an asymmetric manner and the positions of the nuclei define the rostral ends. Subsequently, the nuclei differentiate into needle-shaped structures containing tightly packed DNA and point towards the SV [1,4]. As spermatid elongation proceeds, the rostral ends of the nuclei bundle (NB) advance towards the SV at the basal end of the testis. Then, F-actin-based conical structures called investment cones form around each of the needle-shaped nuclei and shortly thereafter, move down the axoneme at a constant speed [4,5]. This process separately invests each individual spermatid with a plasma membrane, extrudes excess cytoplasm and organelles from the cells, and discharges them as waste bags [3]. Thus individualized, sperm bundles coil up at the base of the testis and then enter the SV [1,4]. F-actin dynamics, myosin VI, dynamin, dynein light chain 1 (DDLC1/LC8) and myosin V [5-9] as well as several pro-apoptotic proteins [10,11] are involved in investment cone assembly and the sperm individualization process. The cyst cells also differentiate along with the spermatids. Two cyst cells of different morphological features are found to encapsulate the spermatid bundle at the start of individualization. The head cyst cell caps the rostral ends like a lid on a tube and the rest of the spermatids are enclosed within the tail cyst cell [1,4]. However, little is known about the molecular basis of sperm release after individualization.

The sperm release process has been extensively studied in the mammalian system. Seminiferous tubules are the functional units of mammalian spermatogenesis. A layer of somatic-origin Sertoli cells line the tubules and constitutes the blood-testis barrier. The Sertoli cells adhere to each other through sets of tight junctions and desmosome-gap junctions [12,13]. The spermatocytes traverse through these sets of inter-cellular junctions from the basal to the adluminal side as they differentiate (stages 1–7), and complete the differentiation (stages 8–16) while still attached to the adluminal side of the Sertoli cell [13-16]. Then they are released as mature sperm into the lumen. This last stage is called spermiation. Prior to this, each spermatid sheds a residual body containing membrane organelles and cytoskeletal elements, which forms at the junction of the sperm head and flagella [14]. This resembles the shedding of waste bag after individualization in Drosophila testis [3]. Spermatids also associate with the Sertoli cells through cell-adhesion complexes. A testes specific adherens junction (AJ) called an ectoplasmic specialization (ES) is formed at the inner side of the Sertoli cell [17]. This contains characteristic hexagonal actin arrays packed between the ER cisterns and the plasma membrane with a dense formation of tubulin fibers adjacent to the ER [18,19]. Such a structure has not been reported in invertebrates [13].

In this paper we report the results of a systematic analysis of the final stages of sperm maturation before their release in Drosophila testis. We show that the somatic-origin head cyst cell grows F-actin based membranous projections into the interstitial spaces between the mature spermatid heads at the start of individualization. Immunohistochemical analysis showed that these F-actin-rich processes contained markers of filopodia and also proteins found in the AJ. Pharmacological manipulations of the F-actin and microtubule dynamics further revealed that these structures are dynamic and are involved in maintaining mature spermatids in a tight parallel bundle. Finally, a genetic screen identified that shibire (dynamin) function is essential to maintain the integrity of these F-actin-based structures and the sperm bundle at the final stage of maturation. Altogether our data provide an initial set of descriptions for further cellular and molecular analysis of spermiation in Drosophila .

Results

F-actin-based membranous extensions of the head cyst cell cover the sperm heads after individualization

An adult testis contains multiple cysts at different stages of differentiation. They are recognized by the nuclear morphology of the spermatids and the relative positions in the testis. For instance, the needle-shaped nuclei of the elongated spermatids are loosely organized in the cyst (*, Figure 1A, Additional files 1 and 2) at the start of individualization and these are generally found at about 250 μm from the base. The F-actin-rich investment cones (also known as F-actin cones) form around each nucleus during the individualization stage and move towards the caudal ends in synchrony [1,3,5,6]. The NBs of the individualizing spermatids (arrowheads, Figure 1A) are found in a region 200–255 μm from the testis base (Figure 1A), and they move further towards the SV in subsequent developmental stages (fine arrows, Figure 1A) until the mature sperm enter the SV. The entire process was estimated to take nearly 20 hours [1,3]. Therefore, the rostral ends of the maturing spermatid bundles are likely to traverse around 250 μm to the SV in as much time or longer.

thumbnailFigure 1. F-actin-rich structures cap the mature nuclei at the beginning of sperm individualization. (A) Wild-type testis stained with fluorescein isothiocyanate (FITC):phalloidin (green) and 4',6-diamidino-2-phenylindole (DAPI) (red), respectively. Spermatid NBs associated with investment cones (*), at the early (arrows) and intermediate (arrowheads) stages of individualization, and of the coiled-up stages (fine arrows) are marked. Rostral ends of the NBs moved very slowly towards the SV; see Additional files 1 and 2. (B), (C) DAPI (blue) and rhodamine isothiocyanate (RITC):phalloidin (red) stained isolated cysts show F-actin organization at the rostral ends of spermatids during individualization. (B) Arrow indicates F-actin (red) accumulation at the rostral ends of spermatids at the beginning of the investment cone (arrowhead) assembly. (C) Overlay of the differential interference contrast (DIC) picture (gray scale) of the isolated NB containing the needle shaped nuclei (blue) and the F-actin (arrows) cap at the rostral ends. (D), (E) Isolated cysts from sneaky-GFP (green) testis strained as above. (D) NB of a post individualized spermatids. Actin caps appeared around the acrosomes marked by sneaky-GFP (arrows). (E) NB of a relatively later stage bundle (post coiling) found at the testis base. F-actin staining disappeared from the rostral ends but increased laterally (arrowhead). This is presumed to be the penultimate stage before the sperm is released. (F) Combined DIC (Grey) and epifluorescence image of the head cyst cell and associated spermatids stained with anti-Lamin (Dm0) (green), DAPI (blue) and RITC: phalloidin (red). The cell perimeter (broken line) and Lamin-rich membrane folds inside the cell (arrows) are marked. Lamin also localized along the F-actin extensions (arrowheads).

Additional file 1. A Three-dimensional rocking movie of the nuclei bundles and actin caps distribution at the base of the wild-type testes. A confocal Z-focus series of an immunostained specimen was collected by using a 40× NA 0.9 objective. The testes preparation was mounted with protective spacers to maintain the original spatial distribution in the tissue. Nuclei were stained with 4',6-diamidino-2-phenylindole (DAPI) (blue) and F-actin was stained with fluorescein isothiocyanate (FITC):phalloidin (green). A corresponding Z-projection of the image stack is shown in Figure 1(A).

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thumbnailAdditional file 2. Movie of the acrosome movements in the wild-type testis in phosphate buffered saline. The time-lapsed images of isolated y w; P [w +mc snky:GFP] testis collected using a 60× (oil) NA 1.42 objective attached to a laser scanning confocal microscope. Each frame was averaged for 2 scans (at around 1 second per scan) and collected every 3 minutes. A total of 20 frames are presented in this movie. The movie runs at two frames per second. The acrosomes of the elongating spermatids (red arrows) were found to move quite well. In comparison, the compacted acrosome bundles were mostly stationary (white arrows). A two-dimensional time projection of these frames is shown in Figure 5(A).

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Additional F-actin accumulations were observed at the rostral tips of the NBs (arrows, Figure 1B) at the start of individualization. Subsequently, the F-actin grew as cap-like structures around the spermatid nuclei during individualization (Figure 1C). The F-actin densities were found around rostral tips of individual nuclei and acrosomes of an NB (Figure 1D), marked by the sneaky-GFP [20]. At a later stage when the individualized and mature sperm coiled up inside the cyst, the F-actin densities were mostly found around the lateral sides of the nuclei (arrowhead, Figure 1E). This is likely to correspond to a stage when sperm were about to be released as both the acrosomes and the NB appeared unpacked (arrow, Figure 1E). These F-actin based structures will be referred as 'actin caps' in the subsequent discussion. The actin caps were also observed to form inside the head cyst cell covering the rostral ends of maturing spermatid bundles (Figure 1F) and occasionally 'empty' actin caps not associated with the NB were also observed inside the testis. These observations raised an obvious question about the cellular origin of the actin caps.

Transmission electron microscopy (TEM) studies further showed that the nuclei of the 'individualized' spermatids were tightly invested with plasma membrane (arrows, Figure 2A) and contained very little cytoplasm. They were embedded into the head cyst cell (HC, Figure 2A) with electron dense material around (fine arrows, Figure 2A; see also the inset). An earlier study reported that membrane bound projections containing microfilaments are extended from the head cyst cell and interspersed between the sperm heads after individualization [4]. We also found some tightly packed NBs (arrows, Figure 2B) with dense material around them near the head cyst cell perimeter (fine arrows, Figure 2B; see also the inset). Together with the previous results this suggested that the actin caps are likely to form inside the head cyst cells and therefore, unlikely to be a part of the spermatids.

thumbnailFigure 2. Transmission electron microscopy images of transverse sections of mature sperm heads inside a cyst at the base of a wild-type testis (magnification 28,000×). (A) Cross-section view of mature and individualized sperm nuclei (arrows) inside the head cyst cell (HC, arrowheads). The stage is determined according to an earlier description [4]. Inset shows enlarged view of the nuclei (N) tightly invested with plasma membrane (white arrowhead). The nuclei are embedded in an electron dense material (fine arrows) and membranous projections filled the interstitial space (marked with *). (B) Nuclei (arrows) of post-individualized spermatids inside the head cyst cell are tightly packed with electron dense material around them (fine arrows). They were also placed at one side of the HC. Inset shows 10× enlarged view of part of the bundle. The interstitial space (*) is packed with electron dense material.

To further establish this point we used two Gal4 stocks, (a) SG18.1Gal4 and (b) pCOGGal4 , which were found to express in the head cyst cells during the final stages of sperm maturation (Additional file 3). Although the SG18.1Gal4 expression was also found in the spermatocytes at an earlier stage, the pCOGGal4 expression was limited to the cyst cells from the very beginning (Additional file 3). The expression of UAS-actin:GFP in SG18.1Gal4 background marked the actin caps (arrows, Figures 3A and 3B) and the F-actin cones (Figure 3C). However, only the actin caps were marked in the pCOGGal4/Y; UAS-actin:GFP/+ testis (arrows, Figure 3D). Furthermore, the recombinant myosin-VII:GFP expression in the w pCOGGal4 UAS-ck:GFP stock clearly marked the actin caps at the rostral ends of mature NBs (arrows, Figure 3E–G). These observations confirmed that the actin caps are indeed formed inside the head cystcells. In addition, the actin caps were always found attached with the spermatid heads in squash preparations. This suggested that these F-actin-based extensions of the head cyst cell are likely to adhere to the maturing sperm heads. However, TEM studies did not reveal obvious cellular junctions between the sperm heads and the cyst cells.

Additional file 3. The Gal4/UAS-reporter expression patterns in the testes. (A) Confocal sections show myosin VII-GFP (green) and myr-mRFP1 localizations in the testis from the w UAS-myosin VII-GFP pCOGGal4/Y; UAS-myr-mRFP1/+ adults. The expression was localized in the germ-line stem cells and primary gonial precursors (arrowheads) at an early stage. Later on, it was contained in the cyst cells (arrows). The UAS-myr-mRFP1 (myristoylated mRFP1) expression generally marks the cell membrane and highlights the cysts cell perimeter (arrows, A") around the spermatocytes in the testis. (B) The UAS-actin:GFP expression in the pCOGGal4/Y; UAS-actin:GFP/+ testis is increased in the head cyst cells (HC, dotted lines) at the final stages before the mature sperm release from the cysts. B-B" indicates different focus levels of the same testis. The rhodamine isothiocyanate (RITC):phalloidin (red) and 4',6-diamidino-2-phenylindole (DAPI) (blue) staining are shown in appropriate false colors. (C) The head cyst cells (arrows) are prominently marked by the combined expression of UAS-GFP and UAS-actin:GFP in SG18.1Gal4 background during the sperm individualization stages.

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thumbnailFigure 3. Actin caps are extended from the head cyst cell. (A) Optical section from the SG18.1Gal4 UAS-GFP/UAS-GFP:actin testes stained with rhodamine isothiocyanate (RITC):phallodin (red) and 4',6-diamidino-2-phenylindole (DAPI) (blue) show GFP and GFP-actin localizations in the head cyst cell nucleus (arrowhead) and in the actin cap (arrows), respectively. (B), (C) A similar staining of the SG18.1Gal4 UAS-GFP:actin/+ testis shows prominent GFP:actin localization in the actin cap (arrows, (B)) and also in the investment cones (C). (D) The actin cap (arrows) is also labeled by GFP:actin in pCOGGal4/Y; UAS-GFP:actin/+ testis. (E)-(G) Confocal sections from the DAPI (blue) stained w pCOGGal4 UAS-myosin VII:GFP/Y testis show myosin-VII:GFP (green) localization around the nuclei bundles (NBs) (arrows, (E)) at the testis base. (F) Higher magnification image of a single NB show prominent myosin VII:GFP localization along the NB (arrowheads) and also in the head cyst cell (arrows). (G) RITC:phalloidin (red) and DAPI (blue) staining further revealed that the myosin VII:GFP is enriched in the actin cap (arrows). The arrowheads indicate an empty actin cap. (See Additional file 3 for a detailed analysis of expression pattern in testes.)

Cell adhesion proteins along with certain filopodial components are enriched in the actin caps

Therefore, to further understand the role of the actin caps, we decided to characterize its molecular composition. Regulation of actin dynamics and AJs are known to play crucial roles in epithelial cell morphogenesis and movement during development. In addition, the basolateral protrusive activity of these cells that penetrate into the neighboring cells to promote the formation of adhesive contacts is suggested to involve actin [21]. Immunostaining revealed that prominent AJ markers such as DE-cadherin and crumbs were enriched in the head cyst cells (arrows, Figure 4A). The Drosophila lamin (Dm0), armadillo (beta-catenin), tubulin and ERp72 were also enriched at the actin caps (arrows, Figure 4B). ERp72, which seemed to be enriched more caudally than the others, is an ER resident protein found in testes specific AJs [22,23]. This suggested that actin caps could adhere to the sperm heads through AJs. Actin-based membrane extensions such as filopodia and invadopodia are also known to require dynamin, syndapin and WASP [19,24-27]. These were also associated with the actin caps (arrows, Figure 4C, a, b and 4C, c). In addition, syntaxin [28], a t-SNARE involved in membrane fusion events, was present along the actin cap extensions (arrows, Figure 4D). Thus, actin caps contained proteins characteristic of both filopodia and AJs. They are likely to adhere to sperm heads through beta-catenin, which is an integral component of AJ in the epithelial cells [29] and testes [19].

thumbnailFigure 4. Immunohistochemical characterization of the actincap. (A) Confocal images of head cyst-cells in intact testis containing mature and individualized sperm nuclei from the basal regions of testes, stained with: ras, DE-cadherin, crumbs and lamin antisera (all in green), as well as rhodamine isothiocyanate (RITC): phalloidin (red) and 4',6-diamidino-2-phenylindole (DAPI) (blue). Arrows indicate staining in the membranous folds inside the head cyst cell and arrowheads indicate the head cyst cell nuclei. (B) Isolated mature spermatids without the head cyst cells as found in the testis squash preparations stained with lamin (Dm0), armadillo, tubulin and ERp72 antisera (indicated at the left side panel of each set). (C), (D) Similar preparations stained with the dynamin (a), syndapin (b) and WASP (c) as well as (D) Syntaxin (green). Arrows indicate positions of the actin caps. (E) Head cyst cells inside testis immunostained with the (a) ck/myosin VII and (b) jar/myosin VI, respectively, or expressing (c) the sqh-GFP and (d) the clathrin light chain:GFP (clc-GFP ), respectively. (a) ck (green) staining (arrows) coincided with the membranous fold and (b) anti-jar stained the actin cap (arrows) as well as a few punctate spots in the cytoplasm (arrowheads). (c) The sqh-GFP localized along the actin caps (arrowheads) and in punctate spots (arrows) in the cytoplasm. (d) clc-GFP mostly localized in the punctate spots (arrows) in the cytoplasm. The sqh-GFP transgene is expressed through its own promoter in sqh homozygous mutant background and the UAS-clc-GFP is expressed by using the actin5CGal4 (see Additional file 6 for details).

Filopodia and pseudopodia require the functions of different unconventional myosins [26,30-33]. We found that both myosin VII (ck) and myosin VI (jar) were present in punctate spots in the head cyst cell cytoplasm (arrows, Figure 4E) and jar/myosin VI was enriched along the actin cap extensions (arrowheads, Figure 4E, b). In addition, expression of the recombinant myosin II regulatory light chain-GFP (sqh-GFP) [34] also marked the actin caps (arrowheads, Figure 4E, c). Myosin VI is known to stabilize F-actin bundles in the microvilli and the stereocilia [33], and both myosin II and VIIa were shown to maintain vesicular traffic into the microvilli projections [33,35]. In Drosophila ovary, myosin VI was localized in the membrane protrusions at the leading edge of migrating border cells [36], and at the investment cones in the testis [6]. Therefore, the actin caps appeared to be filopodia-like projections of the head cyst cells maintained by myosin-dependent vesicular traffic. However, we noted that unlike the ck:GFP, the ck antibody did not label the actin cap extensions. The ck antibody is raised against the stalk domain of the predicted myosin VII ORF [37], which is likely to be involved in homodimerization of the motor subunits and also in binding other accessory subunits. Therefore, unlike the ck:GFP, the antibody could only label myosin VII (ck) in the tissue if the epitope is exposed. Furthermore, the ck:GFP transgene is ectopically expressed using a non-homologues promoter and this could also alter its subcellular localization in the cell. All of these could account for the apparent differences in the subcellular staining patterns of ck in the head cyst cell.

A large number of vesicles were reported to accumulate inside the head cyst cell around the rostral tips of the embedded sperm heads [4]. This was also revealed in the DIC images of the isolated head cyst cells. The punctate distributions of myosins in this region further suggested that these motors might be involved in vesicle transport into the actin cap projections. Myosin VI was known to associate with the clathrin-coated pits inside the cell during endocytosis as well as with dynamic membrane ruffles in the migrating epithelial cells [33]. We found that the clathrin light chain-GFP (clc-GFP) was enriched in punctate spots around the actin caps (arrows, Figure 4E, d) in the w; UAS-clc-GFP Actin5cGal4 testis. Clathrin is involved in coated vesicle assembly [38] and plays an important role in dynamin-mediated vesiculation inside the cell and the clc is an integral part of the clathrin complex. Therefore, this observation further supported the hypothesis that vesicular traffic from the head cyst cells could supply membrane to the actin cap projections. Altogether the immunolocalization results indicated that actin caps are filopodia-like extensions and likely to attach to the sperm heads during individualization.

F-actin and microtubule stability are essential for actin cap assembly and maintenance

Spermatids are maintained in a tight bundle inside the cyst during individualization and this is predicted to play a critical role in selecting out the improperly developed sperm [4]. Live analysis of acrosome movements inside testis by using the sneaky-GFP [20] further showed that the acrosome movements slow down after they are packed (arrows, Figure 5A, Additional files 2 and 4). This coincided with actin cap formation and, thus, suggested that the actin caps are required to maintain the sperm heads in a tight bundle during individualization. Indeed we noticed that few mature nuclei were left out in every NB in almost all wild-type testes during the coiling stage. Both F-actin and microtubule dynamics play important roles in the filopodia/pseudopodia assembly and maintenance [39-41]. Latrunculin B (lat B) binds to actin monomers and prevents their polymerization and the addition of lat B to cultured cells leads to major disruption of actin cytoskeleton [41]. Similarly, treatments with vinblastine (vinb) leads to microtubule depolymerization in the tissue [42,43]. Therefore, to determine the role of the cytoskeletal elements in actin caps, we treated intact testis with these reagents.

thumbnailFigure 5. Mature spermatid heads remain in a tight bundle attached to the testis wall during the coiling stage. (A), (B) Sets of time lapse images of the testis base, expressing the sneaky-GFP transgene [20], were projected together to show the relative movement of the acrosome bundles of a cyst inside the testis. Each frame is labeled with a specific false color as per the list shown in (B). The blue arrows and arrowheads point to the positions of acrosome bundles in the first frame while the white arrows and arrowheads indicate the final position. The arrows indicate the compacted set, which is likely to belong to the post-individualization stages, while the arrowheads indicate the acrosomes of the elongating/pre-individualization stage spermatids. (A) The compacted acrosome bundle (arrows) found near the base of the testis remain confined in the region as indicated by the positions of the blue and white arrow. Some acrosomes (green arrow) are occasionally found to move away from the bundle. This is considered to belong to the defective sperm that are lost during coiling. In comparison the acrosomes of the elongating spermatids (arrowheads) are loosely organized and move rapidly towards the testis base as evident from the positions of the blue and white arrowheads. (B) The mobility of acrosome bundles (blue and white arrows) near the base of the testis increased after 30 minutes of 5 μM vinblastine (vinb ) treatment. (See Additional files 2 and 4 for details.)

thumbnailAdditional file 4. Movie of the acrosome movements in the wild type testis in phosphate buffered saline containing 5 μM vinblastine. The time-lapse images of isolated y w; P [w +mc snky:GFP] testis collected using a 60× (oil) NA 1.42 objective attached to a laser scanning confocal microscope as described for Additional file 5. The movie runs at two frames per second. The testes preparations were treated for 30 minutes in phosphate buffered saline containing 5 μM vinb before imaging. A total of 10 frames are presented in this movie with each frame having its own time stamp. The acrosomes of the elongating spermatids (red arrows) as well as the compacted acrosome bundles (white arrows) appeared to move very slowly. The two-dimensional time projection of these frames is shown in Figure 5(B).

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The acrosome bundles were disrupted and the individual acrosomes moved about each other in the bundles after 30 minutes of the 5 μM vinb treatment (arrows, Figure 5B). A similar result was obtained with the 25 μM lat B as well (data not shown). It also caused severe actin cap disruptions (Figure 6A and Table 1) within 10–30 minutes, with visible loss of F-actin (arrows, Figure 6B and 6C). Although the 5 μM vinb treatment did not abolish the F-actin staining, it disrupted both the actin caps and the NBs (arrow, Figure 6D). These observations suggested that the stability of both the microtubule and F-actin cytoskeleton are essential to maintain the actin caps and this appeared to disrupt the NBs. In addition, the pharmacological treatments also disrupted the F-actin cone organization in the individualization complex (IC) (Figure 6E), although the staining at the F-actin cones was not visibly altered. Finally, a quantification study showed significant increase in the disrupted NB and IC populations after the drug treatments (Figure 6F and 6G), and the disruption indices were strongly correlated in each testis (Figure 6H). The conjugated NB and IC disruptions reflected disorganizations of the spermatid bundles inside the cysts and the rapid manifestation of the defect further suggested that the association between the head cyst cell and the spermatids are dynamic. F-actin disruption in isolated testis of adult moths was shown to block sperm release and spermatid bundle disorganization [44] and studies in mammalian testis showed that the F-actin stability in the apical ES is essential to maintaining association between developing spermatids and Sertoli cells [12].

thumbnailFigure 6. F-actin and microtubule maintain the actin cap, essential to maintain the maturing spermatids in a tight bundle. (A), (B) Wild-type testis stained with 4',6-diamidino-2-phenylindole (DAPI) (blue) and rhodamine isothiocyanate (RITC):phalloidin (red) after 30 minutes in PBS containing, (A) 8 μM DMSO (control), or, (B) 30 μM latrunculin B (lat B). Arrows indicate NBs of the mature sperm associated with the actin caps and arrowheads indicate residual caps left behind after sperm release. SV indicates the direction of the seminal vesicle. (C), (D) Isolated cysts stained with DAPI (blue) and RITC:phalloidin (red) after 30 minutes of incubation in PBS containing either, (a) 8 μM DMSO (control), (b) 30 μM lat B, or (c) 5 μM vinb as indicated at the top of the panels. Corresponding DIC images were overlaid in grey scale (D) to indicate the contours of the sperm heads. (E) F-actin cones of ICs from intact testis treated with (a) DMSO (control), (b) lat B and (c) vinb , respectively. (F), (G) Percentage NB (F) and IC (G) disruptions, after 10 (solid bars) and 30 (open bars) minutes of treatments presented as histograms with +S.E.M., and n > 6 for individual plots. (H) The points in the plot represent statistical correlations between the IC and the NB disruption indices within individual testes after 30 minutes of the lat B treatments. A straight line fitted through the points indicates the probability of a simultaneous occurrence of the two events together as estimated from the observed data (see Table 1 and associated legend for detail).

Table 1. Relative NB and IC disruptions in individual testis after 30 minutes of lat B (25 μM) treatment.

shibire/dynamin is essential for maintaining the actin caps and spermatid bundles

The association between head cyst cells and spermatids was predicted to play an important role in spermiation [4]. The above results indicated that the head cyst cells are likely to adhere to the maturing sperm heads through F-actin-rich projections containing several filopodial markers. Dynamin plays a key role in membrane reorganization process and our immunolocalization data suggested that it could play an important role in actin cap assembly or maintenance as well. The shibire gene codes for a dynamin homologue in Drosophila , which is shown to play an important role in endocytosis [45] and various other cellular functions [46]. Previous studies have also shown that shi ts1 (a conditional mutant allele of shibire ) hemizygous flies rapidly paralyze within a few minutes at non-permissive temperatures [47]. Thus, the conditional shi ts1 alleles provided a good tool to further test the role of dynamin in actin caps and the latter's role in sperm maturation/release.

Testes from the shi ts1 hemizygous males grown at 18°C had no apparent defect in NB organization and actin cap morphology (Figure 7A). However, some of the actin caps (white arrowheads, Figure 7B, b) and a few NBs (red *, Figure 7B, c) appeared disrupted after 30 minutes at 29°C. In addition, a few actin caps were found without the associated nuclei (red arrowheads, Figure 7B, b). Interestingly, F-actin levels in the disrupted actin caps were not reduced (Figure 7C), indicating that shibire/dynamin functions were required to maintain the attachments to sperm heads. In addition, there was a small but significant increase in the numbers of mature NBs at the testis base after the heat treatment (Figure 7D). Since the gonial precursors form at an hourly rate, cysts are expected to mature at the same rate. Therefore, the small increase in the NBs after a 30 minute pulse is quite significant. This indicated that shibire could regulate premature sperm release. This would also suggest that the sperm maturation process is very dynamic and the shibire/dynamin function is constitutively required to maintain the association between the sperm heads and the head cyst cell. However, this failed to resolve whether the shibire/dynamin acts at the actin caps.

thumbnailFigure 7. Actin caps and NBs are disrupted in hemizygous shibire mutant testes after heat pulse. (A), (B) Wild type (A) and hemizygous shi ts1 mutant (B) testes labeled with FITC:phalloidin (green) and 4',6-diamidino-2-phenylindole (DAPI) (blue) after 30 minutes at 29°C. Regions populated by the coiled-up sperm bundles are marked by the dotted lines. Note that the base (arrows) is slightly enlarged in shi ts1 hemizygous testis. Figures (b), (c) of (A) as well as (B) are enlarged views of the regions marked in (a) of (A), and (a) of (B), respectively. The figures in the (c) of A and (B) indicate the DAPI labeled NBs. The white arrowheads indicate actin caps and arrows indicate the NBs in respective figures. The red star indicates a highly disrupted NB and red arrowhead indicates abnormal actin caps in figures (b) and (c) of (B). (C) Isolated cysts stained with RITC: phalloidin (red) and DAPI (blue) from (a, b) wild type control and (c, d) the shi ts1 hemizygous mutant testes before and after 30 minutes at 29°C. Arrows indicate actin caps in figures (c) and (d). (D) Histograms indicate average NBs in a testis in wild type and shi ts1 adults after 30 minutes of incubation at 18°C (grey filled bars) and at 29°C (open bars), respectively. The error bars indicate ± S. E. M. The number of specimen (n) for each bar and the pair wise test of significance (p values) are indicated on each figure. The non-significant (ns), significant (*) and very significant (**) differences are indicated on each set of bars linked by the horizontal lines.

shibire/dynamin function is required in the head cyst cells to maintain the actin caps and the sperm head bundles

The ectopic expression of the recombinant UAS-shi ts1 transgene was shown to disrupt the endogenous shibire functions at non-permissive temperature (29°C) in a dose-dependent dominant-negative manner [48]. We used this technique to temporally perturb the shibire/dynamin function in the head cyst cells. The UAS-shi ts1 expression in the head cyst cells owing to the pCOGGal4 as well as the SG18.1Gal4 drivers caused visible actin cap and NB disruptions within 30 minutes at 29°C (Figure 8A and 8B, Additional file 5). In addition, F-actin rich spots appeared to accumulate in the head cyst cell after the heat pulse (arrowheads, Figure 8A and 8B). Same set of Gal4 driver stocks were used to drive UAS-WASP ΔCA and UAS-GFP expressions in the head cyst cells as controls. The recombinant WASPΔCA lacks 30 amino acids in the C-terminal part constituting the VCA domain of the wild-type protein and therefore, it cannot bind to Arp2/3 [49]. Its expression in the Drosophila myoblasts caused fusion defects [50]. However, expressions of the UAS-WASP ΔCA (Figure 8C) or UAS-GFP in the head cyst cells did not disrupt the actin caps even after the heat pulse. These observations further established that the effects observed after the heat pulse due to the ectopic UAS-shi ts1 expression in the head cyst cells are indeed caused by the presence of recombinant shibire(ts1) protein, and further proved that the shibire function is required in the head cyst cells to maintain the actin cap morphology and its attachment with the spermatid heads. Since the shibire antigen along with syndapin is enriched near the actin caps, this could further suggest that dynamin function is required in the actin cap region. Therefore, sihibire/dynamin mediated membrane dynamics in the actin cap region is likely to play an important role in maintaining adhesion between the maturing sperm heads and the head cyst cell, and thus prevent premature release of spermatids inside the testis.

thumbnailFigure 8. Transient disruption of the shibire function in the head cyst cell caused actin cap and nuclei bundle disruptions. The UAS-shi ts1 expression in the head cyst cells due to the (A) pCOGGal4 and (B)SG18.1Gal4 drivers, respectively, disrupted the actin caps and nuclei bundles (NBs) at non-permissive temperature (29°C). Rhodamine isothiocyanate (RITC):phallidin and 4',6-diamidino-2-phenylindole (DAPI) staining of these testes (a) before and (b) after the heat pulse showed visible disruptions of the actin caps and NBs (arrows). In addition, there was punctate accumulation of F-actin in the head cyst cell cytoplasm (arrowheads) after the heat pulse. Fine arrows indicate mature nuclei separated from the actin caps. This is not found in the wild-type controls treated in a similar manner. The actin cap and the NB morphology remained unaltered even after 30 minutes at 29°C in the pCOGGal4 UAS-GFPck/Y and SG18.1 UAS-GFP/UAS-actin:GFP testes (data not shown). (C) The UAS-WASP ΔCA expressions by using the pCOGGal4 caused no detectable NB disorganization and a mild accumulation F-actin rich spots in the cytoplasm after a 30 minute heat pulse. (See Additional file 5 for further details.)

Additional file 5. Transient disruption of the shibire function in the head cyst cell caused actin cap and NB disruptions. The UAS-shi ts1 expression in the head cyst cells due to the (A) pCOGGal4 and (B) SG18.1Gal4 drivers, disrupted the actin caps and nuclei bundles (NBs) at non-permissive temperature (29°C). Rhodamine isothiocyanate (RITC):phalloidin and 4',6-diamidino-2-phenylindole (DAPI) staining of these testes before (a), (b) and after (c), (d) the heat pulse showed visible disruptions of the actin caps and NBs (arrows). In addition, there was punctate accumulation of F-actin in the head cyst cell cytoplasm (arrowheads) after the heat pulse. Fine arrows indicate mature nuclei separated from the actin caps. This is not found in the wild-type controls treated in a similar manner. The actin cap and the NB morphology remained unaltered even after 30 minutes at 29°C in the pCOGGal4 UAS-GFPck/Y and SG18.1Gal4 UAS-GFP/UAS-actin:GFP testes (data not shown). (C) The UAS-WASP ΔCA expressions by using (a), (b) pCOGGal4 and (c)-(e) SG18.1Gal4 caused mild loss of F-actin staining from the actin caps but no detectable NB disruptions even after a 30 minute heat pulse.

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Discussion

In summary, our results highlight the cellular and molecular mechanism of the sperm bundling process in Drosophila . Previous anatomical studies in Drosophila [4] as well as in several other insects [51,52] established that spermatids are tightly bundled towards the end of differentiation and before their release into the SV. This process is predicted to single out abnormal sperm after individualization and thus acts as a quality control step in spermiogenesis [1,4]. However, the cellular and molecular mechanism underlying this process was unknown. The sperm heads are embedded into the somatic-origin head cyst cell at the start of individualization. We have shown that they are held by the head cyst cell through F-actin-based extensions (Figure 9A), Immunohistochemical characterizations combined with pharmacological interventions showed that these are filopodia-like extensions adherent to the maturing sperm heads (Figure 9B). Finally, molecular genetic analysis suggested that shibire/dynamin function is essential in the head cyst cells to maintain the sperm heads in a bundle and prevent their premature release inside the testis. These observations have several interesting parallels with the spermiation process in the mammalian system as discussed in the following sections.

thumbnailFigure 9. Anatomical organization and molecular compositions of actin cap structures around the sperm head assembly inside head cyst cell. (A) Summary of the observations and indication of the positions of (a) the individualization stage, (b) coiled-up stage and (c) empty cysts inside a testis (gray out line). The positions of germ line stem cell (GSC) at the apex and the head and tail cyst cells are marked with distinct colors as indicated in the figure. The axoneme of maturing spermatids (black), the cell nuclei (blue) and major F-actin based structures (red) are highlighted to illustrate the basic observation of this study. The rostral ends of the sperm nuclei moved very slowly towards seminal vesicle during individualization until the mature sperm penetrate the head cyst cell to enter the seminal vesicle. (B) Cross-section view of the relative organizations of actin caps inside head cyst cell and sperm heads during individualization. It is partly adapted from a previous description [1] and updated to summarize the data presented in this report. Only four spermatids are drawn for simplicity in illustrating the organization. The compositions of the different regions are indicated by color coding as shown in the adjacent panel.

Actin caps organize spermatid heads in a tight bundle inside the head cyst cell

Post individualization, all of the 64 clonally derived spermatids are twisted together and the bundle coils up inside the cyst. The head cyst cell attaches to a terminal epithelial cell at the base of the testis, and then the sperm is released into the SV [4]. Our studies identified a specific function of the head cyst cell in the sperm bundling process during the maturation stage. As summarized in Figure 9B, it is found to grow several F-actin-based extensions at the start of individualization that are enriched with proteins found in the filopodia of other cell types [53]. In addition to dynamin, syndapin and WASP, proteins found in the apical ES [54,55] such as ERp72, E-cadherin and beta-catenin are enriched in the actin cap extensions. This suggests that the actin cap extensions are dynamic membrane bound projections and they adhere to the individualized sperm heads while the remaining parts of the spermatids undergo morphogenetic changes during the individualization and coiling stages. Lamin, a protein associated with the F-actin cones during the sperm individualization process [10] was enriched at these structures. This could strengthen the membrane cytoskeleton and provide rigidity to the actin cap extensions. Myosins are essential for the formation of filopodia-like extensions [33] and for cell adhesion [56,57] in several other cell types. Hence, the presence of the myosin II regulatory light chain, Myosin VI and myosin VII suggests frequent vesicular membrane transport into and out of the actin cap projections as well as cross linking of actin filaments to the plasma membrane inside these extensions. The presence of clathrin light chain and syntaxin further pointed towards the existence of dynamic membrane exchange along the actin cap projections. Altogether these immunolocalization studies suggested that actin cap extensions could adhere to the maturing sperm heads during individualization and coiling through a combination of different cellular mechanisms. This is also confirmed by the results of our pharmacological and genetic analyses, which indicates that the actin cap extensions and their interactions with the spermatid heads are dynamic. Altogether, it showed that the differentiating spermatids are tightly attached to the head cyst cell until they are fully mature and suggested that this could play a critical role in preventing premature sperm release inside the testis.

F-actin-based membrane remodeling at filopodia and pseudopodia-like structures is involved in cell migration, axonal growth cone guidance and cell ingestion [58]. In Drosophila ovary, the somatic origin 'border cells' migrate through the germ-line 'Nurse cells' by extending similar F-actin-based membranous projections [59]. Actin-based cellular extensions were also known to anchor epithelial cells on the substratum involving beta-catenin and cadherin [36]. White blood cells migrate out of the blood vessel by penetrating the endothelial cell layer through such actin-based membranous projections by a process called transcytosis [16]. Drosophila spermatids lose all of their cytoplasmic contents after individualization by the movement of the F-actin-based investment cones through them [3,7]. Hence, the somatic-origin cyst cells that encapsulate these near-mature sperm are expected to play an important role in regulating their release. This is further established by a targeted disruption of shibire/dynamin functions in the head cyst cells, which indicate that the maintenance of spermatids in a tight bundle is essential for preventing a premature release. This is also consistent with observations made in the mammalian testis. The Sertoli cells play an important role in the mechanical movement of germ cells from the basal to the adluminal side during their differentiation [12]. This process is aided by the F-actin-rich ES and a variety of different cellular junctions [14-16]. Our results helped to identify the molecular and cellular framework involved in the spermiation process in Drosophila . This also provides a useful base for future analysis of sperm release mechanisms.

Dynamin-mediated membrane remodeling is essential for actin cap maintenance and attachment to sperm nuclei at the terminal stages of maturation

Dynamin is implicated in the assembly and maintenance of F-actin-based membrane ruffles, podosomes and invadopodia [60-63], and a specific dynamin isoform in rat (dyn3) was found to associate with the tubulobulbar junctions around the sperm heads [64,65]. Dynamin is enriched at the actin caps and genetic analysis showed that they are essential for maintaining the sperm bundle inside the cysts. The dynamin requirement appeared to be constitutive as the temporal loss of shibire/dynamin function caused actin cap disassembly and NB disorganization. In addition, it caused accumulation of NBs inside the testes within 30 minutes. Thus, dynamin-mediated membrane remodeling at the actin cap region is expected to play a role in sperm bundling and release processes in Drosophila , and provided a basis to investigate the interactions between the somatic origin cyst cells and spermatids during the sperm maturation process in Drosophila.

Conclusion

In view of the observations presented here, the sperm maturation process in Drosophila resembled the spermatid development inside the Sertoli cell layer in mammalian testis [14]. The mammalian spermatids attach to the Sertoli cell membrane through the apical ES [66]. The F-actin-based ES forms around developing spermatids inside the Sertoli cell [13]. Although electron microscopic studies did not reveal ES-like structures around the sperm heads in Drosophila testis, the actin caps contained the essential functional elements of these specialized junctions. It is rich in F-actin, beta-catenin and DE-cadherin, and tightly associated with the spermatid heads during individualization. In addition, we found a second interesting parallel. Mammalian sperm are physically released from the Sertoli cells after the removal of integrin and beta-catenin from the apical ES [67]. A tubulobulbar junction forms inside the Sertoli cells and around the mammalian spermatids [54,55]. Proteins involved in endocytosis, such as dynamin 3 and amphiphysin were enriched at these junctions [64,65,68], and loss of amphiphysin from the tubulobulbar junctions was shown to block sperm release in the knockout mice [68]. We showed that shibire/dynamin functions in the head cyst cells in Drosophila testis to maintain the actin cap integrity and sperm heads in a tight bundle. Thus, our observations have the potential to establish Drosophila as an attractive model for molecular analysis of spermiation in insects. It has defined an assay to study the role of F-actin-mediated cell adhesion process and can be used to screen for small molecule-based perturbation of the sperm maturation process in the future.

Methods

Drosophila stocks and culture conditions

All of the fly stocks used for this study are listed in Additional file 6. They were maintained on standard cornmeal agar medium as described previously [46]. We sincerely acknowledge the generous gifts of fly stocks from the respective sources. For most of the immunostaining and analysis, 2-day old adult males grown at room temperature were used. The conditional mutant alleles of shibire/dynamin (shi ts1 and shi ts2 ) were grown at 18°C and 2-day old adults were shifted to either 29°C or 32°C for a defined period before dissection and immunostaining. All of the transgenic fly stocks were grown at 25°C. The crosses set for the heat pulse studies were grown at 18°C. Subsequently, the freshly emerged males were maintained at the same temperature for 2 days before they were shifted to the non-permissive temperatures as per the requirements.

Additional file 6. Table S1. Table listing Drosophila melanogaster stocks used in this study

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Immunohistochemistry

For whole mount analysis, testes were dissected in phosphate buffered saline (PBS) [69] containing 0.01% saponin (Sigma Chemical Co., MO, USA), incubated in the same solution for 30 minutes and then fixed in PBS containing 4% paraformaldehyde (freshly prepared). After several quick rinses in PTX (PBS with 0.3% Triton X-100), the fixed testes were incubated in different primary antibody solutions in PTX for 1 hour at room temperature. This was followed by washes in PTX, and further incubation in appropriate fluorescent labeled secondary antisera in PTX for 1 hour. After a final series of washes, the tissue preparations were mounted on a glass slide with a drop of Vectashield® (Vector Laboratories Inc., USA) and under a #1 cover glass. To observe the nuclei and the F-actin distribution in the tissue, the immunostained specimen were additionally incubated in PTX containing 76 μM of FITC:phalloidin or RITC:phalloidin (Sigma Chemical Co., MO, USA) and 0.001% DAPI (Sigma Chemical Co., MO, USA) for 30 minutes, washed in PTX and then mounted as above. In some cases individual cysts were teased out of the testis after staining and dispersed on the slide before mounting. This helped to reveal the subcellular distribution of F-actin structures. For squash preparations, the testes were dissected on a plus charged slide, squashed under a cover slip, dipped in liquid nitrogen for a few minutes and then in ice-cold 95% ethanol for 3 minutes, or, until the cover slip dropped off. This was followed by a post fixation of the slide in PBS containing 4% paraformaldehyde and immunostaining as per the procedure described earlier [46]. This often disrupted the head cyst cells but kept the NBs intact. In all of these cases the actin caps were found to remain associated with the intact NBs.

For the pharmacological treatments, adult testes were dissected in PBS containing 0.003% DMSO and either 30 μM lat B or 5 μM vinb (both from Sigma Chemical Co., MO, USA), and then incubated in the same media for 30 minutes before fixation. Then they were processed as described above. For short heat pulses, dissected testes in PBS were incubated in a water bath set at defined temperatures (18°C for controls and 29°C for heat pulse) for the specified period of time. Then they were processed as described above.

A list of all of the antibodies used for this study and their respective sources is provided in Additional file 7. All of the fluorescently conjugated secondary antibodies were obtained from the Jackson Research Laboratories Inc., USA, and from the Molecular Probes Inc., OR, USA, and used at 1:400 dilutions.

Additional file 7. Table S2. Table listing the antibodies used in this study

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Electron microscopy

Testes were dissected from 2-day old males in 2.5% glutaraldehyde (EM Sciences Inc., USA), 4% paraformaldehyde and 0.04% CaCl2 in 0.1 M phosphate buffer (pH 7.4) at 4°C, then fixed overnight in the same solution, washed in 0.1 M phosphate buffer (pH 7.4) and post-fixed in OsO4 for 4 hours at 4°C. This was followed by several washes in 0.1 M phosphate buffer (pH 7.4), dehydration in graded series of ethanol, and embedding in Araldite (E. Merk GmbH, Germany). Ultrathin (100 nm) sections were obtained in Leica Ultracut 6b, stained with aqueous uranyl acetate and lead citrate, and imaged in a JEOL 100S electron microscope as per the procedure described previously [46].

Statistical analyses of the NB and IC morphologies in the wild type and mutants

The number of mature NBs and the ICs were counted in each testis preparation under a 40 × 0.75 NA objective fitted in an epifluorescence microscope. The NBs and the ICs were carefully scrutinized for abnormal organizations and if they were found to be out of register with each other, then they were counted as disrupted. The principal criteria used for this analysis has been described earlier [8]. Briefly, the criterion for a NB to be considered as intact, most of the nuclei should remain in parallel register and be packed tightly together (example: Figure 6C, a). Only those which appeared obviously disrupted were counted as not intact (example: Figure 6C, b and 6C, c). The F-actin cones in an IC are found in a parallel register (example: Figure 6E, a) while the disrupted ones had them scattered to different extents (example: Figure 6E, b and 6E, c). Volunteers also counted some of the preps, selected at random, in a double blind manner. This showed that the criteria used for counting were quite robust. The results were presented as histogram plots with ± standard error of the mean, and the significance of the differences was calculated by using the Mann-Whitney non-parametric test using the Graphpad Instat™ software.

Correlation analysis

The NB and IC defects as well as the total numbers were recorded from 10 lat B treated testes. The relative NB and IC disruption values from individual testis were plotted on the x - and y -axis, respectively. The data was then analyzed by using Graphpad Instat™ and the SigmaPlot™. Graphpad Instat™ was used to determine the pairwise significance (p ) values and to estimate whether the slope was significantly different from zero. SigmaPlot™ was used to determine the slope of the best-fitted line amongst the points in the plot. For studying the correlation between different genotypes, the average NB and IC disruption values (determined as a percentage of the total) from each genotype were plotted and analyzed as described above.

Image collection and analysis

All images were collected by using the Olympus FV1000SPD laser scanning confocal microscope (LSCM). The image frames were merged by using ImageJ® http://rsb.info.nih.gov/ij webcite, and adjusted for their brightness and contrast to maintain uniform visibility in a montage by using Adobe Photoshop® (Adobe Corp., USA). The figures were then organized and labeled in Adobe Illustrator™.

Abbreviations

AJ: adherence junction; ck: crinkled; clc: clathrin light chain; DAPI: 4',6-diamidino-2-phenylindole; DIC: differential interference contrast; ER: endoplasmic reticulum; ES: ectoplasmic specialization; FITC: fluorescein isothiocyanate; GFP: green fluorescent protein; IC: individualization complex; jar: jaguar; lat B: latrunculin B; mRFP1: monomeric red fluorescent protein; NB: nuclei bundle of maturing spermatids; RITC: rhodamine isothiocyanate; shi: shibire; sqh: spaghetti-squash; SV: seminal vesicle; TEM: transmission electron microscopy; UAS: upstream activating sequence; vinb: vinbalstine; WASP: Wiskott-Aldrich syndrome protein.

Authors' contributions

BSD made the initial observation, designed and executed the majority of the experiments, analyzed the data, composed the figures and helped in writing the manuscript. KR participated in the experimental design in collaboration with BSD and executed the final set of experiments, analyzed the data, composed the figures and the manuscript, and supervised the whole project. SS performed the electron microscopy of wild-type testis and provided the TEM data.

Acknowledgements

We thank Barbara Wakimoto, Dan Kiehart, John Roote, and Eyal Schejter for generous and prompt sharing of fly stocks and reagents. We thank Vimlesh Kumar and Mani Ramaswamy for providing the syndapin antibody. P. Koshire helped KR in setting up genetic crosses and in sample preparations. BSD and KR also acknowledge the summer students, D. Kar, A. Sarkar and K. Mukherjee, who helped in the initial stages of this project. BSD thanks A. Ghosh Roy for valuable discussions. Research described in this article was supported by an intramural grant from TIFR, India to KR and a TIFR scholarship to BSD.

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