Table 1 |
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|
Primers used for polymerase chain reaction and reverse transcriptase polymerase chain reaction amplification. |
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|
Gene |
Primer |
Sequence (5' to 3') |
|
|
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|
Δ14 |
Δ14-ORF-s |
CCCAGCAAACATGGCAGACATAGAC |
|
Δ14-ORF-as |
CGCGAAACTTCTACGCACCGTAACTT |
|
|
Δ14-5'RACE |
CGCTCATGTCAATGGTCTTCCTCAGCTT |
|
|
Δ14-3'RACE |
ATGAACAAAGGAGACGGCACTTACGAGG |
|
|
|
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|
Δ11 |
Δ11-5'RACE |
GCCAAGCACAGCAAGATTCAGAAGAACG |
|
Δ11-3'RACE |
GATTGGAATCACTGCTGGTGCCCATAGA |
|
|
Δ11-RT-s |
GGCATTTCAAAATACTGCGCTCTCGTGG |
|
|
|
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|
16S rRNA |
16S-s |
TGAAGGGCTGCAGTATTTTG |
|
16S-as |
TCGAGGTCGCAAACTCTTTT |
|
|
|
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Lassance and Löfstedt BMC Biology 2009 7:10 doi:10.1186/1741-7007-7-10 |
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