Figure 5.

Activated cPLA₂ is associated with PrP^Sc in ScGT1 cells. (A) Immunoblots showing amounts of cPLA₂ immunoprecipitated with antibodies to PrP (4F2 or IC18), CD55 or an isotype control (Iso) from untreated ScGT1 cells (Untreated) or from ScGT1 cells pre-treated with 0.2 units PI-PLC for 30 minutes (PI-PLC). (B) The amounts of activated cPLA₂ (expressed as a percentage) in whole cell extracts from ScGT1 cells (open bars), from ScGT1 cell extracts depleted with the anti-PrP mab 4F2 (black bars) and in 4F2 immunoprecipitates (IPT) from ScGT1 cell extracts (striped bars). Values shown are the mean ± SD from 10 samples.