Figure 1.

Analysis of the protein products synthesized in vitro from 4.1R cDNAs. A. Schematic representation of the exon map for the 4.1R protein. Exons are coded as follows: striped, alternative; white, constitutive; black, non-coding. The number of each exon is shown at the bottom. Two translation-initiation sites at exons 2' (ATG1) and exon 4 (ATG2) are indicated, as is the stop codon (TGA) at exon 21. B and C, exon map of the 4.1R¹³⁵ cDNAs (B) and the 4.1R⁸⁰ cDNAs (C) used in the in vitro-coupled transcription and translation assays. D. In vitro-coupled transcription and translation reactions of the indicated 4.1R cDNAs. Reaction products were labelled with [³⁵S] methionine and autoradiographed. A control reaction containing all components of the mixture except the cDNA template is also shown (-).