Figure 5.

Gel electrophoresis of PCR products amplified from individual fly genomic DNAs. In each gel, DNA size standards are in outermost lanes, interior lanes contain products obtained from four individual Hsp70^-, Hsp70^A-Ba-, Hsp70⁺ and Hsp70^traIII flies as marked. (A) 1 kb Hsp70 fragment, comprising the 3' half of the Hsp70 CDS. (B) 1 kb fd3F fragment. (C) 140 bp Hsp70 fragment, from the 3' end of the CDS, amplified with a 60°C annealing step; same reaction employed in quantitative PCR. (D) 104 bp RpL32 CDS fragment amplified with a 60°C annealing step; same reaction employed in quantitative PCR. (E) 193 bp Hsp70 fragment, from the 5' end of the CDS, amplified with primers specific to Hsp70Aa/Ab. (F) 193 bp Hsp70 fragment, from the 5' end of the CDS, amplified with primers specific to Hsp70Ba/Bb/Bbb/Bc.