Table 1

Activity of tmRNA -1 triplet mutants in the KanR assay
UUU X
 UCU X
 UAU X
 UGU X
UUC 10
 UCC 100
 UAC 25
 UGC 100
UUA X
 UCA 35
 UAA X
 UGA X
UUG X
 UCG 100
 UAG 100
 UGG 50
CUU X
 CCU X
 CAU 40
 CGU X
CUC 50
 CCC 50
 CAC 100
 CGC 60
CUA X
 CCA 100
 CAA X
 CGA X
CUG 100
 CCG 100
 CAG 90
 CGG 20
AUU 90
 ACU X
 AAU 90
 AGU 100
AUC 100
 ACC 100
 AAC 80
 AGC 100
AUA 60
 ACA 100
 AAA 100
 AGA 100
AUG 100
 ACG 100
 AAG 100
 AGG 100
GUU 90
 GCU X
 GAU 100
 GGU 100
GUC 100
 GCC 100
 GAC 100
 GGC 100
GUA X
 GCA X
 GAA 95
 GGA 100
GUG 100
 GCG 25
 GAG 100
 GGG 100

tmRNA directs the addition of the last 15 amino acids of KanR onto ribosomes stalled on a kanR template in Escherichia coli. Only cells with functional tmRNA survive on 30 μg/ml kanamycin [11]. Each -1 triplet mutant was tested individually and the data reported as percentage survival on selective (kanamycin) media. Triplets listed as X showed no survival. Triplets forbidden by the rules developed by Lim and Garber [16] are underlined and in bold type.

Miller et al. BMC Biology 2008 6:29   doi:10.1186/1741-7007-6-29