Additional file 1:

Table of normalized expression of nonredundant genes with symbols in ovary and testis.

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Additional file 2:

Principal Component Analysis (PCA) of expression of all genes in ovary (A) and testis (B) of mice from 1 to 24 months old on ad libitum (AL) or calorie restriction (CR) diet. In ovary, PC1 is associated with age; PC2 is related to an initial increase of gene expression followed by a decrease in the oldest age; PC3 represents the effect of calorie restriction, which is strongest at the age of 6 months. In testis, PC1 is related to age; PC2 is associated with CR but mostly in the oldest animals. The first two principal components accounted for 58% of variance in testis compared to 74% in ovary, which indicates that PCA patterns for testis were noisier than those for ovary due to very limited changes in gene expression values.

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Additional file 3:

Table of genes that were differentially expressed between ovary and testis. Genes with at least two-fold over-expression in ovary (or testis) are called ovary – (or testis) associated genes.

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Additional file 4:

Table of genes with differential expression in ovary between young (1–6 months) and old (16–24 months) females on an AL diet.

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Additional file 5:

Table of GO annotations of genes with differential expression in ovary between young (1–6 months) and old (16–24 months) females on and AL diet.

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Additional file 6:

Gene expression changes in mouse ovarywith age from 1 to 24 months on ad libitum (AL) or calorierestriction (CR) diet. Panels A-F show genes whose expression increases with age; panels G-L show genes whose expression decreases with age. Genes were selected arbitrarily to represent each functional category, but most of them were genes with the greatest differential expression in each functional category. (A) interleukin-related genes, (B) MHC-II genes, (C) complement component genes, (D) C-lectin genes, (E) apoptosis-related genes, (F) TNF receptor genes, (G) oogenesin genes, (H) zona pellucida genes, (I) other oocytes-specific genes, (J) cell cycle-related genes, (K) BMP-signaling genes, and (L) DNA repair genes.

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Additional file 7:

Table of genes with differential expression in testis between young (1–6 months) and old (16–24 months) males on an AL diet.

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Additional file 8:

Gene expression changes in mouse testis with age from 1 to 24 months on ad libitum (AL) and calorie restriction (CR) diet. Panels A and B show genes whose expression increases with age; panels C and D show genes whose expression decreases with age. Genes were selected arbitrarily to represent each functional category, but most of them were genes with the greatest differential expression in each functional category. (A) immune response genes; (B) metabolism genes; and (C) extracellular matrix genes.

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Additional file 9:

Table of GO annotations of genes with differential expression in testis between young (1–6 months) and old (16–24 months) males on an AL diet.

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Additional file 10:

Table of genes affected by calorie CR in ovary (ages 6–24 months combined, age effects subtracted).

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Additional file 11:

Table of the effect of CR on the expression of oocyte-specific genes in ovary (ages 6–24 months combined).

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Additional file 12:

Table of GO annotations of genes affected by CR in ovary (ages 6–24 months combined).

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Additional file 13:

Gene expression changes in mouse ovary (A-F) and testis (G-I) with ad libitum (AL) or calorie restriction (CR) diet. Genes were selected arbitrarily to represent each functional category, but most of them were genes with the greatest differential expression in each functional category. (A) circadian rhythm genes, (B) metabolism regulating genes, (C) transporter activity genes, (D) lipid/steroid metabolism-related genes, (E) cell adhesion genes, (F) electron transport genes, (G) testis-associated genes, (H) aging-related gene, and (I) interferon responsive gene.

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Additional file 14:

Table of genes affected by CR in ovary at specific age.

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Additional file 15:

Table of genes affected by CR in testis (ages 6–24 months combined, age effects subtracted).

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Additional file 16:

Table of genes affected by CR in testis at specific age.

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Additional file 17:

Table of the expression of genes associated with aging, DNA repair, and heat shock response in ovary and testis.

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Additional file 18:

Expression of genes associated with aging and DNA damage control in ovary and testis in mice from 1 to 24 months old on ad libitum (AL) or calorie restriction (CR) diet. Genes were selected arbitrarily to represent each category. (A) genes involved in human diseases, (B) sirtuin family genes, and (C) telomerase-related genes.

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Additional file 19:

Table of primers for q-PCR.

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Additional file 20:

Quantitative RT-PCR analysis of selected genes in ovary and testis in mice from 1 month to 24 months on AL or CR diet.

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