|
Properties of hue-shifted mTFP1 variants |
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| Excitation maximum (nm) |
Emission maximum (nm) |
ε(mM-1 cm-1) |
Φ |
Brightnessa (mM-1 cm-1) |
pKa |
Photostabilityb |
|
|
|
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| mTFP1-Y67W |
424/440c |
461/482c |
13 |
0.02 |
0.3 |
NDd |
ND |
| mTFP1-Y67H |
369e |
NAf |
7 |
NA |
NA |
ND |
NA |
| G1 |
487 |
503 |
43 |
0.60 |
26 |
ND |
ND |
| G2 |
487 |
503 |
60 |
0.65 |
39 |
ND |
65 |
| G3 |
498 |
515 |
70 |
0.70 |
49 |
ND |
5.5 |
| mWasabi |
493 |
509 |
70 |
0.80 |
56 |
6.5 |
93 |
|
|
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| mTFP1g |
462 |
492 |
64 |
0.85 |
54 |
4.3 |
110 |
| EGFPh |
488 |
507 |
56 |
0.60 |
34 |
6.0 |
174 |
|
aProduct of extinction coefficient and quantum yield. bTime in seconds to bleach from an initial emission rate of 1000 to 500 photons s-1 molecule-1. cValues for both 'humps' are provided. dND, not determined. eThis value is the absorption maximum. No significant fluorescence was detected for mTFP1-Y67H. fNA, not applicable. gValues from [19]. hValues from [7]. | |||||||
Ai et al. BMC Biology 2008 6:13 doi:10.1186/1741-7007-6-13 |
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