Contributions of chaperone and glycosyltransferase activities of O-fucosyltransferase 1 to Notch signaling
1 Nagoya University Graduate School of Bioagricultural Sciences, Department of Applied Molecular Biosciences, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan
2 Howard Hughes Medical Institute, Waksman Institute and Department of Molecular Biology and Biochemistry, Rutgers The State University of New Jersey, Piscataway, NJ 08854, USA
BMC Biology 2008, 6:1 doi:10.1186/1741-7007-6-1Published: 14 January 2008
Additional file 1:
Distribution of various ER markers in the wing disks. Vertical section is shown with apical up in all panels. (A) UAS-iOfut1 [16.2]; ptc-Gal4 wing disk immunostained with OFUT1 (green) and Calnexin (magenta). This line exhibits only partial silencing of OFUT1 expression, visible on the left-hand side of the panel. (B), (C) Immunostaining of wild-type wing disks with (B) Boca (green) and Calnexin (magenta) or (C) OFUT1 (green) and KDEL (magenta). (D), (E) Wing disks expressing GFP:KDEL (magenta) under ptc-Gal4 control are immunostained with (D) Boca or (E) OFUT1. (F), (G) UAS-iOfut1 [12.3]; ptc-Gal4 wing disks raised at 29°C are immunostained with Notch (magenta) and (F) KDEL or (G) Boca (green). The dashed line marks the edge of the ptc expression stripe.
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