Representative CO2 emission traces for the four species used in this study. The x-ray beam (25 keV, 80 μW/mm2) was incident on the insect's head. No qualitative changes are seen immediately after beam on. A major change in CO2 emission pattern after 1000–1500 s of x-ray exposure is marked by RS (respiratory signature). The RS was based on CO2 emission patterns and was corroborated with x-ray video behavioral data; the RS is a major change in CO2 release pattern associated with shaking of the head or mouthparts. For the grasshoppers (Schistocerca gregaria), no behavioral change was observed at RS.
Socha et al. BMC Biology 2007 5:6 doi:10.1186/1741-7007-5-6