Extensive population genetic structure in the giraffe1 Department of Ecology and Evolutionary Biology, University of California, Los Angeles, CA, 90095, USA 2 Center for Conservation and Research, Omaha's Henry Doorly Zoo, 3701 South 10th Street, Omaha, NE 68107, USA 3 Mpala Research Centre, PO Box 555, Nanyuki, Kenya
BMC Biology 2007, 5:57doi:10.1186/1741-7007-5-57
Additional filesAdditional file 1: Table showing giraffe sampling localities and sample sizes for mtDNA characterization with resulting mtDNA control region haplotypes Format: DOC Size: 58KB Download file This file can be viewed with: Microsoft Word Viewer Additional file 2: Figure showing maximum parsimony phylogeny of giraffe (Giraffa camelopardalis) mtDNA haplotypes, rooted with okapi (Okapia johnstoni) Format: DOC Size: 25KB Download file This file can be viewed with: Microsoft Word Viewer Additional file 3: Figure showing maximum parsimony phylogeny of giraffe (Giraffa camelopardalis) mtDNA haplotypes, rooted using midpoint rooting Format: DOC Size: 25KB Download file This file can be viewed with: Microsoft Word Viewer Additional file 4: Figure showing minimum evolution phylogeny of giraffe (Giraffa camelopardalis) mtDNA haplotypes, rooted with okapi (Okapia johnstoni) Format: DOC Size: 26KB Download file This file can be viewed with: Microsoft Word Viewer Additional file 5: Figure showing minimum evolution phylogeny of giraffe (Giraffa camelopardalis) mtDNA haplotypes, rooted using midpoint rooting Format: DOC Size: 26KB Download file This file can be viewed with: Microsoft Word Viewer Additional file 6: Figure showing maximum likelihood phylogeny of giraffe (Giraffa camelopardalis) mtDNA haplotypes, rooted with okapi (Okapia johnstoni) Format: DOC Size: 26KB Download file This file can be viewed with: Microsoft Word Viewer Additional file 7: Figure showing maximum likelihood phylogeny of giraffe (Giraffa camelopardalis) mtDNA haplotypes, rooted using midpoint rooting Format: DOC Size: 25KB Download file This file can be viewed with: Microsoft Word Viewer Additional file 8: Table of AMOVA results according to subspecific groupings Format: DOC Size: 47KB Download file This file can be viewed with: Microsoft Word Viewer Additional file 9: Table of sample sizes, number of mitochondrial haplotypes and molecular diversity indices per sampling locality and subspecies Format: DOC Size: 81KB Download file This file can be viewed with: Microsoft Word Viewer Additional file 10: Table of sampling locations (six historical subspecies, 30 sample sites, 381 individuals) for microsatellite characterization Format: DOC Size: 90KB Download file This file can be viewed with: Microsoft Word Viewer Additional file 11: Table of summary statistics for microsatellite data (381 specimens, all populations and pelage subspecies) Format: DOC Size: 38KB Download file This file can be viewed with: Microsoft Word Viewer Additional file 12: Tables showing (A) observed and expected heterozygosity, and deviations from Hardy-Weinberg equilibrium in six giraffe subspecific populations, and (B) observed and expected heterozygosity, and deviations from Hardy-Weinberg equilibrium in 16 giraffe populations Format: DOC Size: 281KB Download file This file can be viewed with: Microsoft Word Viewer Additional file 13: Tables showing (A) posterior probability population assignments of 381 Individuals, based on assignment to pelage/subspecies designations using STRUCTURE [19], and (B) subspecies assignment of 381 individuals, based on assignment to pelage/subspecies designations, using multilocus genotypes and Bayesian analysis (Rannala and Mountain method in Geneclass2 [46,47]) Format: DOC Size: 42KB Download file This file can be viewed with: Microsoft Word Viewer Additional file 14: Table of STRUCTURE [23] cluster results identify three possible subspecies hybrids, four population hybrids within the same subspecies and one possible population migrant within the same subspecies Format: DOC Size: 44KB Download file This file can be viewed with: Microsoft Word Viewer Additional file 15: Figure showing STRUCTURE [19] cluster assignments of detected giraffe hybrids Format: DOC Size: 149KB Download file This file can be viewed with: Microsoft Word Viewer Additional file 16: Tables showing (A) pairwise Fst values and statistical significance for the six giraffe subspecies; (B) pairwise values for Nei's genetic distance among six giraffe subspecies; (C) G.c. angolensis (Angolan) population pairwise comparison Fst values and statistical significance; (D) G.c. reticulata (Reticulated) population pairwise comparison Fst values and statistical significance; (E) G.c. rothschildi (Rothschild's) population pairwise comparison Fst values and statistical significance; (F) G.c. tippelskirchi (Masai) population pairwise comparison Fst values and statistical significance; and (G) G.c. tippelskirchi (Masai) Serengeti N.P. population pairwise comparison Fst values and statistical significance Format: DOC Size: 95KB Download file This file can be viewed with: Microsoft Word Viewer Additional file 17: Table showing migration rates among giraffe subspecies Format: DOC Size: 40KB Download file This file can be viewed with: Microsoft Word Viewer Additional file 18: Table of primer sequences and amplification characteristics of Giraffa camelopardalis microsatellite locus NECK484 Format: DOC Size: 28KB Download file This file can be viewed with: Microsoft Word Viewer Additional file 19: Figure showing likelihood values for inferred number of genetic clusters (K) from STRUCTURE [23] (three iterations per value of K) Format: DOC Size: 54KB Download file This file can be viewed with: Microsoft Word Viewer Additional file 20: Table of overall Fis values per subspecies, and per population Format: DOC Size: 60KB Download file This file can be viewed with: Microsoft Word Viewer Additional file 21: Table of Mantel test of isolation by distance results (correlation of genetic distance (Fst/(1-Fst) with geographic distance) Format: DOC Size: 24KB Download file This file can be viewed with: Microsoft Word Viewer Additional file 22: Table of AMOVA using microsatellite data results Format: DOC Size: 55KB Download file This file can be viewed with: Microsoft Word Viewer |




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