 Research articleDynacortin facilitates polarization of chemotaxing cellsCathryn Kabacoff1 1 Department of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA 2 Department of Electrical and Computer Engineering, Johns Hopkins University, Baltimore, MD, USA 3 Current address: The Henry M. Jackson Foundation for the Advancement of Military Medicine, VPRP/DAIDS/NIAD, 6700-A Rockledge Drive, Room 42A165, Bethesda, MD, USA 4 Current address: Human Genome Sciences, Inc. 14200 Shady Grove Road, Rockville, MD, USA
BMC Biology 2007, 5:53doi:10.1186/1741-7007-5-53
Additional filesGFP dynacortin concentrates along the cortex and enriches at the leading edge during chemotaxis. Video is imaged using wide-field epifluorescence with a 40 × (NA 1.3) objective and a 1.6× optivar. Frames are queued every 15 s. Format: AVI Size: 839KB Download file Playing the movie within this page requires QuickTime 3 or later and JavaScript. Read more GFP dynacortin concentrates along the surface at the leading edge and at sites of cell protrusions during chemotaxis. Video is imaged using total internal reflection fluorescence microscopy using a 60 × (NA 1.45) 1.6× optivar. Frames are queued every 1 s. Format: AVI Size: 725KB Download file Playing the movie within this page requires QuickTime 3 or later and JavaScript. Read more GFP dynacortin concentrates along the surface at the leading edge and at sites of cell protrusions during chemotaxis. Video is imaged using total internal reflection fluorescence microscopy using a 60 × (NA 1.45) 1.6× optivar. Frames are queued every 1 s. Format: AVI Size: 970KB Download file Playing the movie within this page requires QuickTime 3 or later and JavaScript. Read more Wild-type (Ax2) control (carrying the empty vector) cells become highly polarized and move smoothly towards a needle injecting 1 μM cAMP. Video is imaged using differential interference contrast imaging with a 40 × (NA 1.3) objective and a 1× optivar. Frames are queued every 5 s. Format: AVI Size: 1.6MB Download file Playing the movie within this page requires QuickTime 3 or later and JavaScript. Read more Wild-type (Ax3(Rep orf+)) control (carrying the empty vector) cells become highly polarized and move smoothly towards a needle injecting 1 μM cAMP. Video is imaged using differential interference contrast imaging with a 40 × (NA 1.3) objective and a 1× optivar. Frames are queued every 5 s. Format: AVI Size: 2.4MB Download file Playing the movie within this page requires QuickTime 3 or later and JavaScript. Read more Wild-type (Ax2):dynhp cells do not become highly polarized and have trouble moving towards the needle injecting 1 μM cAMP. Video is imaged using differential interference contrast imaging with a 40 × (NA 1.3) objective and a 1× optivar. Frames are queued every 5 s. Format: AVI Size: 1.6MB Download file Playing the movie within this page requires QuickTime 3 or later and JavaScript. Read more Wild-type (Ax3(Rep orf+)):dynhp cells do not become highly polarized and have trouble moving towards the needle injecting 1 μM cAMP. Video is imaged using differential interference contrast imaging with a 40 × (NA 1.3) objective and a 1× optivar. Frames are queued every 5 s. Format: AVI Size: 2.4MB Download file Playing the movie within this page requires QuickTime 3 or later and JavaScript. Read more Wild-type (Ax2:pLD1) control cell showing the skeleton as it moves towards the needle located to the left of the field. The skeleton is computed and displayed at each frame (green bar). Extending pseudopods are marked with red tips and retracting pseudopods are marked with blue tips. Note that the green skeleton represents the shape of the cell in the current frame (frame n). The red tips are generated from the difference between the n and n + 1 frames of the video. Because the red tips mark extending pseudopods, they will not link to the green skeleton in the first frame that they appear but will link in the subsequent frame. The blue tips are also generated from the difference between the n and n + 1 frames but because they are retracting they always link to the green skeleton. Video is imaged using differential interference contrast imaging with a 40 × (NA 1.3) objective and a 1× optivar. Frames are queued every 5 s. Format: AVI Size: 12.7MB Download file Playing the movie within this page requires QuickTime 3 or later and JavaScript. Read more Wild-type (Ax2): dynhp cell showing the skeleton as it moves towards the needle located to the bottom of the field. The skeleton is computed and displayed at each frame (green bar). Extending pseudopods are marked with red tips and retracting pseudopods are marked with blue tips. Here the cell sends out multiple pseudopods at a time and at a variety of angles. Note that the green skeleton represents the shape of the cell in the current frame (frame n). The red tips are generated from the difference between the n and n + 1 frames of the video. Because the red tips mark extending pseudopods, they will not link to the green skeleton in the first frame that they appear but will link in the subsequent frame. The blue tips are also generated from the difference between the n and n + 1 frames but because they are retracting they always link to the green skeleton. Video is imaged using differential interference contrast imaging with a 40 × (NA 1.3) objective and a 1× optivar. Frames are queued every 5 s. Format: AVI Size: 6.3MB Download file Playing the movie within this page requires QuickTime 3 or later and JavaScript. Read more |
|
|
|
 |  | Log on / register Feedback | Support | My details |
on Google Scholar
author email
corresponding author email|
|
| © 1999-2009 BioMed Central Ltd unless otherwise stated. Part of Springer Science+Business Media. |