Additional file 1.

Principles of tomographic reconstruction. The sample (blue) has been cleared to allow light to pass through. Light passes through the tissue depending on the depth and density of the tissue. The image collected of the light passing through the sample is a projection image, as used in OPT. If projection images are collected at multiple angles as the sample is rotated the shape of the object can be reconstructed. On the right-hand side of the image the projection images are back projected and as more angles are added they create the shape of the original sample. In this example, 19 different angles are used. Back projection is the simplest method of reconstruction and many of the evident artefacts are removed using the algebraic methods utilized for OPT reconstruction.

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Bryson-Richardson et al. BMC Biology 2007 5:34   doi:10.1186/1741-7007-5-34