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A 100%-complete sequence reveals unusually simple genomic features in the hot-spring red alga Cyanidioschyzon merolae

Hisayoshi Nozaki1 email, Hiroyoshi Takano2 email, Osami Misumi3,4 email, Kimihiro Terasawa5,6 email, Motomichi Matsuzaki1 email, Shinichiro Maruyama1,6 email, Keiji Nishida3,4 email, Fumi Yagisawa3,4 email, Yamato Yoshida7,4 email, Takayuki Fujiwara3,4 email, Susumu Takio8 email, Katsunori Tamura6 email, Sung Jin Chung2,10 email, Soichi Nakamura9 email, Haruko Kuroiwa3,4 email, Kan Tanaka6 email, Naoki Sato5 email and Tsuneyoshi Kuroiwa3,4 email

Department of Biological Sciences, Graduate School of Science, the University of Tokyo, Tokyo, Japan

Graduate School of Science and Technology, Kumamoto University, Japan

Department of Life Science, College of Science, Rikkyo (St. Paul's) University, Tokyo, Japan

Research Information Center for Extremophile, Rikkyo (St. Paul's) University, Tokyo, Japan

Department of Life Sciences, Graduate School of Arts and Sciences, the University of Tokyo, Tokyo, Japan

Institute of Molecular and Cellular Biosciences, the University of Tokyo, Tokyo, Japan

Department of Integrated Biosciences, Graduate School of Frontier Sciences, the University of Tokyo, Chiba, Japan

Center for Marine Environment Studies, Kumamoto University, Kumamoto, Japan

Laboratory of Cell and Functional Biology, Faculty of Science, University of the Ryukyus, Okinawa, Japan

10  Radiation Research Center for Bio-Technology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeollabuk-do, Korea

author email corresponding author email

BMC Biology 2007, 5:28doi:10.1186/1741-7007-5-28

Published: 10 July 2007

Additional files

Additional file 1:

Supplementary methods: Cloning and sequencing of terminal regions of all chromosomes, and complete determination of the histone cluster area in C. merolae.

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Additional file 2:

Table 2. Primers used for determination of terminal sequences of C. merolae chromosomes.

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Additional file 3:

Figure 5. Telomere length analyses. (A) Southern hybridisation using the probe specific for the left arm of chromosome 15, demonstrating that the left end of chromosome 15 was detected using genomic DNA digested with each enzyme. H, Sa and Sp indicate genomic DNA digested with HindIII, SalI and SphI, respectively. (B) Comparison of the detected signal size with the fragment size estimated from the genome sequences.

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Additional file 4:

Table 3. Primers used for completing the C. merolae histone cluster area.

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