Research article
A 100%-complete sequence reveals unusually simple genomic features in the hot-spring red alga Cyanidioschyzon merolae
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* Corresponding author: Hisayoshi Nozaki nozaki@biol.s.u-tokyo.ac.jp
1 Department of Biological Sciences, Graduate School of Science, the University of Tokyo, Tokyo, Japan
2 Graduate School of Science and Technology, Kumamoto University, Japan
3 Department of Life Science, College of Science, Rikkyo (St. Paul's) University, Tokyo, Japan
4 Research Information Center for Extremophile, Rikkyo (St. Paul's) University, Tokyo, Japan
5 Department of Life Sciences, Graduate School of Arts and Sciences, the University of Tokyo, Tokyo, Japan
6 Institute of Molecular and Cellular Biosciences, the University of Tokyo, Tokyo, Japan
7 Department of Integrated Biosciences, Graduate School of Frontier Sciences, the University of Tokyo, Chiba, Japan
8 Center for Marine Environment Studies, Kumamoto University, Kumamoto, Japan
9 Laboratory of Cell and Functional Biology, Faculty of Science, University of the Ryukyus, Okinawa, Japan
10 Radiation Research Center for Bio-Technology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeollabuk-do, Korea
BMC Biology 2007, 5:28 doi:10.1186/1741-7007-5-28
Published: 10 July 2007Additional files
Additional file 1:
Supplementary methods: Cloning and sequencing of terminal regions of all chromosomes, and complete determination of the histone cluster area in C. merolae.
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Additional file 2:
Table 2. Primers used for determination of terminal sequences of C. merolae chromosomes.
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Additional file 3:
Figure 5. Telomere length analyses. (A) Southern hybridisation using the probe specific for the left arm of chromosome 15, demonstrating that the left end of chromosome 15 was detected using genomic DNA digested with each enzyme. H, Sa and Sp indicate genomic DNA digested with HindIII, SalI and SphI, respectively. (B) Comparison of the detected signal size with the fragment size estimated from the genome sequences.
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Additional file 4:
Table 3. Primers used for completing the C. merolae histone cluster area.
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