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EST library IDs categorized by the description of developmental stages. Each categorized mouse developmental stage contained about 22,300 (409–71,504) EST counts on average, which corresponded to 2,900 (194–5,923) non-redundant ENSEMBL gene entries on average. Developmental status was based on Manipulating the Mouse Embryo) [27] and The Mouse [28]. |
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| Stage |
Developmental status |
dbEST library ID |
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|
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| 1 |
Unfertilized egg |
16178, 16177, 10029, 14142, 1389, 867 |
| 2 |
Fertilized egg, 1 cell |
12233, 2589, 12241, 1119, 17903 |
| 3 |
2 Cell |
862, 1382, 5391 |
| 4 |
4 Cell |
12243, 15709, 1524, 7328 |
| 5 |
8 Cell |
9845, 15708, 1381 |
| 6 |
Morula |
12244, 1532, 18029 |
| 7 |
Blastocyst, E4.0 |
12245, 10026, 1310, 1021, 875, 850, 18027, 12246, 18021 |
| 8 |
Trophoblast, ES cell |
10033, 10023, 14556, 915, 10025, 10024, 14558 |
| 9 |
Formation of egg cylinder (E5.0) |
12231, 18022 |
| 10 |
Proamniotic cavity in primitive ectoderm (E6.0) |
2565 |
| 11 |
Advanced endometrial reaction, gastrulation starts, embryonic axis determined (E6.5) |
13851, 10561 |
| 12 |
Primitive streak, amnion forming (E7.0) |
5422, 547, 2381, 375, 2790, 12316 |
| 13 |
Neural plate, presomite stage (E7.5) |
10036, 3777, 10562, 844 |
| 14 |
Neural folds, otic placode, 1–7 somites, hindgut pocket, 1st pharyngeal arch (E8.0) |
2566, 1913 |
| 15 |
Neural folds close, 8–12 somites, 1st and 2nd pharyngeal arch, turning of embryo (E8.5) |
10039, 538, 4042 |
| 16 |
Formation and closure of anterior neuropore, olfactory placode, 13–20 somites (E9.0) |
5432, 7256 |
| 17 |
Forelimb bud, 21–29 somites (E9.5) |
5392, 13850 |
| 18 |
Hindlimb and talbud, 30–34 somites (E10) |
1884, 2517, 16788 |
| 19 |
Deep lens pit, 35–39 somites (E10.5) |
1891, 15706, 528 |
| 20 |
Closure of lens vesicle, 40–44 somites (E11.0) |
1885, 4142, 12312, 2557, 2578, 9944, 18018 |
| 21 |
Forefoot plate, lens vesicle detached (E11.5) |
15707, 845 |
| 22 |
Hind foot plate, earliest sign of fingers (E12.0) |
5319, 9846, 5464, 5423, 2514 |
| 23 |
Anterior footplate indented, (E13.0) |
12258, 2521, 2554, 2521, 5469, 2555 |
| 24 |
Skin layers, chondrification of ribs (E13.5) |
532, 15702 |
| 25 |
Fingers separate distally (E14.0) |
12259, 2525 |
| 26 |
Fingers & toes separate (E15.0) |
17914, 2596, 12265, 2522, 17951, 2594 |
| 27 |
Rib ossification (E15.5) |
529, 12979 |
| 28 |
Reposition of umbilical hernia (E16.0) |
12260, 2523, 18085, 12247 |
| 29 |
Wrinkled skin, umbilical hernia gone (E17.0) |
12261, 18071, 12250, 12248, 12251, 2516, 1907 |
| 30 |
Auditory ossicles (E17.5) |
12267, 18087 |
| 31 |
Iris and ciliary body (E18.0) |
12266, 1770, 12313 |
Irie and Sehara-Fujisawa BMC Biology 2007 5:1 doi:10.1186/1741-7007-5-1 |
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