Multiple, non-allelic, intein-coding sequences in eukaryotic RNA polymerase genes

doi:10.1186/1741-7007-4-38

BMC Biology

Volume 4

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Research article

Multiple, non-allelic, intein-coding sequences in eukaryotic RNA polymerase genes

Timothy JD Goodwin , Margaret I Butler and Russell TM Poulter

Department of Biochemistry, University of Otago, P.O. Box 56, Dunedin, New Zealand

author email corresponding author email

BMC Biology 2006, 4:38doi:10.1186/1741-7007-4-38


Published:	27 October 2006

Additional files

Additional File 1:

An alignment of RNA polymerase sequences. Taken from accession data as described in the Methods section.

Format: ADDI Size: 83KB Download file

Additional File 2:

The nucleotide sequence and three-frame conceptual translation of the putative RNA polymerase from P. ramorum. The RNA polymerase protein sequence is shaded in red and the intein sequence in blue.

Format: DOC Size: 89KB Download file

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Additional File 3:

The genomic context of the intein-coding sequence in Phytophthora ramorum. The diagram depicts the structures of contigs 4, 5 and 6 of scaffold 19 of the assembled P. ramorum genome sequence. ORFs are represented by the shaded boxes. Blue-shaded boxes represent ORFs having a high quality match (E<1 × 10^-30) in the assembled Phytophthora sojae genome sequence (see 4). Red-shaded boxes represent ORFs whose best matches among all the protein sequences in GenBank are proteins coded by African swine-fever virus (4). The intein and associated RNA polymerase are represented by ORFs 6, 7 and 8 of contig 5. ORFs are as determined by the ORF finder program http://www.ncbi.nlm.nih.gov/gorf/gorf.html webcite, except ORF1 of contig 4, which was extended back to the previous stop codon. Contig 6 extends further than the sequence depicted here.

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Additional File 4:

Matches to the ORFs in P. ramorum scaffold 19, contigs 4, 5 and 6.

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Additional File 5:

An alignment of the intein splicing domains used to create Figure 5.

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