Multiple, non-allelic, intein-coding sequences in eukaryotic RNA polymerase genes
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* Corresponding author: Margaret I Butler margi.butler@stonebow.otago.ac.nz
Department of Biochemistry, University of Otago, P.O. Box 56, Dunedin, New Zealand
BMC Biology 2006, 4:38 doi:10.1186/1741-7007-4-38
Published: 27 October 2006Additional files
Additional File 1:
An alignment of RNA polymerase sequences. Taken from accession data as described in the Methods section.
Format: ADDI Size: 83KB Download file
Additional File 2:
The nucleotide sequence and three-frame conceptual translation of the putative RNA polymerase from P. ramorum. The RNA polymerase protein sequence is shaded in red and the intein sequence in blue.
Format: DOC Size: 89KB Download file
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Additional File 3:
The genomic context of the intein-coding sequence in Phytophthora ramorum. The diagram depicts the structures of contigs 4, 5 and 6 of scaffold 19 of the assembled P. ramorum genome sequence. ORFs are represented by the shaded boxes. Blue-shaded boxes represent ORFs having a high quality match (E<1 × 10-30) in the assembled Phytophthora sojae genome sequence (see 4). Red-shaded boxes represent ORFs whose best matches among all the protein sequences in GenBank are proteins coded by African swine-fever virus (4). The intein and associated RNA polymerase are represented by ORFs 6, 7 and 8 of contig 5. ORFs are as determined by the ORF finder program http://www.ncbi.nlm.nih.gov/gorf/gorf.html webcite, except ORF1 of contig 4, which was extended back to the previous stop codon. Contig 6 extends further than the sequence depicted here.
Format: EPS Size: 54KB Download file
Additional File 4:
Matches to the ORFs in P. ramorum scaffold 19, contigs 4, 5 and 6.
Format: DOC Size: 58KB Download file
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Additional File 5:
An alignment of the intein splicing domains used to create Figure 5.
Format: TXT Size: 16KB Download file
