Guanidine stimulates oligomerization of AβMRF. sup35Δ cells expressing the indicated constructs were grown in the absence (-) or presence (+) of 6.3 mM guanidine (Gu). Equal amounts of lysate proteins were treated with 1% SDS and analyzed by immunoblotting with anti-RF or anti-Aβ antibodies following electrophoresis in agarose. Equal protein loading on each panel was confirmed by coomassie staining of the membrane (not shown).
Bagriantsev and Liebman BMC Biology 2006 4:32 doi:10.1186/1741-7007-4-32