Table 1

Translocation of DAF-16 to cytoplasm in ageing worms. Adult worms containing the muIs71 DAF-16a::GFP transgene and either wild type or the hx546 mutant allele at the age-1 locus were kept in isolated cohorts at 20°C and scored for DAF-16::GFP localisation using a fluorescent compound microscope. Nuclei were scored as either distinctly visible or not distinguishable from the cytoplasm. See Figure 6 for images.

age-1 allele

No. of worms

DAF-16 localisation

DAY 2

DAY 4/5

DAY 6/7

DAY 8/9

DAY 11

DAY 14

wild type

distinct nuclei

28

13

5

1

nuclei not visible

0

12

8

16

age-1(hx546)

distinct nuclei

18

19

6

29

14

13

nuclei not visible

0

0

0

0

0

0

Weinkove et al. BMC Biology 2006 4:1   doi:10.1186/1741-7007-4-1