Table 1

Wnt-5 heterozygous embryos are more sensitive to wnt-5 MO injection in pancreatic islet formation.

WT

WT X wnt-5/WT

trans-block wnt-5 MO

exon-intron wnt-5 MO

trans-block wnt-5 MO

exon-intron wnt-5 MO

% of embryos with abnormal insulin expression

5 ± 2%

0%

46 ± 5%

52 ± 3%

total # of embryos

N = 124

N = 113

N = 53

N = 57

2 ng of either wnt-5 translation-blocking or exon-intron junction targeting morpholinos were injected into either wild-type embryos or embryos generated from an outcross of a wnt-5 mutant carrier adult. In wild-type embryos, injection of either wnt-5 MO resulted in less than 5% of embryos with scattered insulin expression. However, injection of either wnt-5 MO resulted in about 50% of embryos with scattered insulin expression in embryos obtained from the wnt-5 mutant carrier outcross.

Kim et al. BMC Biology 2005 3:23   doi:10.1186/1741-7007-3-23