Wnt5 signaling in vertebrate pancreas development

doi:10.1186/1741-7007-3-23

BMC Biology

Volume 3

Viewing options:

Abstract
Full text
PDF (3.8MB)
Additional files

Associated material:

Related literature:

Articles citing this article
on BioMed Central
on Google Scholar
on ISI Web of Science
on PubMed Central
Other articles by authors
on Google Scholar

Kim HJ
Schleiffarth JR
Jessurun J
Sumanas S
Petryk A
Lin S
Ekker SC

on PubMed

Kim HJ
Schleiffarth JR
Jessurun J
Sumanas S
Petryk A
Lin S
Ekker SC
Related articles/pages
on Google

on Google Scholar

on PubMed
Evaluation of this article
in F1000 Biology

Tools:

Post to:

Research article

Wnt5 signaling in vertebrate pancreas development

Hyon J Kim¹^,2 , Jack R Schleiffarth³ , Jose Jessurun⁴ , Saulius Sumanas² , Anna Petryk¹^,3 , Shuo Lin² and Stephen C Ekker¹

¹Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, MN 55455 USA

²Department of Molecular, Cellular, and Developmental Biology, University of California, Los Angeles, Los Angeles, CA 90095 USA

³Department of Pediatrics, University of Minnesota, Minneapolis, MN 55455 USA

⁴Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, MN 55455 USA

author email corresponding author email

BMC Biology 2005, 3:23doi:10.1186/1741-7007-3-23


Published:	24 October 2005

Additional files

Additional File 1:

(QuickTime format) WT movie. Figure 1C–H panels were taken from this movie at the indicated time points. GFP-positive cells are first observed around the 14 somite stage as bilateral patches in insulin:GFP transgenic embryos. These cells divide and actively migrate to the posterior. The net movement towards the midline is an indirect result of convergent-extension since active migration towards the midline is not observed. By the 24 hpf stage, GFP-positive cells are coalesced into a single islet in the posterior and midline relative to the starting position.

Format: MOV Size: 1.7MB Download file

Playing the movie within this page requires QuickTime and JavaScript. Read more

Additional File 2:

Two color time-lapse imaging shows that GFP-positive cells migrate relative to the neighboring cells. (A) GFP-positive cells are first visible posterior to rhodamine-labeled cells as bilateral lows of cells. (B-G) GFP-positive cells migrate posteriorly and medially, whereas rhodamine-labeled cells do not change their relative position. (H) At 24 hpf, all GFP-positive cells coalesced into a single islet, but the rhodamine-labeled cells remain separated at their original position. Arrows: rhodamine-labeled cells; t: time (minutes).

Format: JPEG Size: 2.5MB Download file

Additional File 3:

(QuickTime format) FZ-2 morphant movie. Figure 1I–N panels were taken from this movie at the indicated time points. As also noted in non-injected insulin:GFP transgenic embryos, GFP-positive cells are first observed around the 14 somite stage as bilateral patches, and these cells divide normally in fz-2 MO-injected embryos. However, the uniform migration towards the posterior observed in the non-injected transgenic embryo is not observed in fz-2 MO-injected embryos. Instead, the polarity of cell migration is entirely lost, with no net movement along the anterior-posterior axis observed for these cells (see text).

Format: MOV Size: 1.4MB Download file

Playing the movie within this page requires QuickTime and JavaScript. Read more

Additional File 4:

Genotyping of embryos with normal and abnormal insulin expression. (A) 90% of embryos with abnormal insulin expression are carriers of ppt^hi1780b. Lane 4 and lane 15 represent wild-type embryos with abnormal insulin expression. (B) Control PCR for exon 1 of wnt-5, N = water only control. (C) None of the embryos with normal insulin expression are carriers of ppt^hi1780b. (D) Control PCR for exon 1 of wnt-5, N = water only control.

Format: JPEG Size: KB Download file