Localization of the cytoplasmic translation and mitochondrial import machinery relative to newly-made mitochondrial transcripts. Cells were grown in BrU for 30 min, fixed, Br-RNA plus different targets immunolabelled, and confocal images collected. Under the conditions used, little Br-RNA made in the nucleus has time to reach the cytoplasm . (A-C) Three views of one field; newly-made mitochondrial Br-RNA is found near S6, a constituent of the cytoplasmic ribosome (shown as red and green in the merge in (C), respectively). (D) The high Pearson's coefficient (rP) at Δx values close to zero confirms that Br-RNA is often found near S6. (E-L) As (A-D); newly-made mitochondrial Br-RNA is also found near the α subunit of NAC (a cytoplasmic chaperone), and Tom22 (a component of the mitochondrial import machinery). Bar: 1 μm.
Iborra et al. BMC Biology 2004 2:9 doi:10.1186/1741-7007-2-9