mtDNA associates with tubulin and a kinesin subunit (KIF5B). Cells with YFP-tagged mitochondria were fixed, various targets immunolabelled and imaged in the confocal microscope. (A-D) Four views of one region of the cytoplasm; mtDNA foci are found in mitochondria that are generally aligned with microtubules (mtDNA and tubulin are pseudocoloured green and red in the merge in (D)). Bar: 2 μm. (E-G) Three views of one region of the cytoplasm; mtDNA foci often lie near KIF5B (pseudocoloured red and green in the merge in (G)). Bar: 1 μm. (H) The high Pearson's coefficient (rP) at Δx values close to zero indicates that mtDNA lies near KIF5B.
Iborra et al. BMC Biology 2004 2:9 doi:10.1186/1741-7007-2-9