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Open Access Highly Accessed Research article

Basal jawed vertebrate phylogeny inferred from multiple nuclear DNA-coded genes

Kanae Kikugawa1, Kazutaka Katoh1, Shigehiro Kuraku12, Hiroshi Sakurai3, Osamu Ishida3, Naoyuki Iwabe1 and Takashi Miyata1*

Author Affiliations

1 Department of Biophysics, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan

2 Laboratory for Evolutionary Morphology, Center for Developmental Biology (CDB), RIKEN, Kobe 650-0047, Japan

3 Tokyo Sea Life Park, Tokyo 134-8587, Japan

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BMC Biology 2004, 2:3  doi:10.1186/1741-7007-2-3

Published: 11 March 2004

Abstract

Background

Phylogenetic analyses of jawed vertebrates based on mitochondrial sequences often result in confusing inferences which are obviously inconsistent with generally accepted trees. In particular, in a hypothesis by Rasmussen and Arnason based on mitochondrial trees, cartilaginous fishes have a terminal position in a paraphyletic cluster of bony fishes. No previous analysis based on nuclear DNA-coded genes could significantly reject the mitochondrial trees of jawed vertebrates.

Results

We have cloned and sequenced seven nuclear DNA-coded genes from 13 vertebrate species. These sequences, together with sequences available from databases including 13 jawed vertebrates from eight major groups (cartilaginous fishes, bichir, chondrosteans, gar, bowfin, teleost fishes, lungfishes and tetrapods) and an outgroup (a cyclostome and a lancelet), have been subjected to phylogenetic analyses based on the maximum likelihood method.

Conclusion

Cartilaginous fishes have been inferred to be basal to other jawed vertebrates, which is consistent with the generally accepted view. The minimum log-likelihood difference between the maximum likelihood tree and trees not supporting the basal position of cartilaginous fishes is 18.3 ± 13.1. The hypothesis by Rasmussen and Arnason has been significantly rejected with the minimum log-likelihood difference of 123 ± 23.3. Our tree has also shown that living holosteans, comprising bowfin and gar, form a monophyletic group which is the sister group to teleost fishes. This is consistent with a formerly prevalent view of vertebrate classification, although inconsistent with both of the current morphology-based and mitochondrial sequence-based trees. Furthermore, the bichir has been shown to be the basal ray-finned fish. Tetrapods and lungfish have formed a monophyletic cluster in the tree inferred from the concatenated alignment, being consistent with the currently prevalent view. It also remains possible that tetrapods are more closely related to ray-finned fishes than to lungfishes.