Additional file 4: Figure S4..
Quantification of labeled cells in sclerotome. (A) Overexpression of Id2, Id3, Foxc2 and Snail1 enhances the proportion of cells encountered in the sclerotome. Refers to Figure 3. (B) Knockdown of Id2/3 and FoxC2. dsRNA to Id2/3 reduce the proportion of labeled cells in sclerotome. Refers to Figure 4A-D. (C) Knockdown of Snail1 reduces the proportion of labeled cells in sclerotome. Refers to Figure 4E-G. In all cases, GFP + cells in sclerotome were negative for desmin and smooth muscle actin. Results are expressed as mean ± SEM. **P ≤0.01. dsRNA, double-stranded RNA; SEM, standard error of the mean.
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Applebaum et al. BMC Biology 2014 12:53 doi:10.1186/s12915-014-0053-9