Additional file 2: Figure S2..

Late activation of Notch in lateral DM-derived progenitors is not sufficient for upregulating SM markers while in the ventral sclerotome. (A,A’) A Tet-GFP-control plasmid was activated twenty hours post-electroporation to the lateral DM for a period of eight hours. Note that the majority of GFP + cells still reside in the lateral DM and only a few delaminated into the intermediate zone between DM and myotome or into the ventral sclerotome (arrows) (N = 9). (B,B’) A Tet-aN2-GFP plasmid was activated twenty hours post-electroporation to the lateral DM for a period of eight hours. Most cells delaminated from the lateral DM and are located in the myotome as desmin/SMA-negative cells (B’) or in the ventral sclerotome (arrows) (N = 8). In both control and Notch-treated cells located in the ventral sclerotome no ectopic SMA/desmin immunostaining is evident. Hoechst nuclear staining is in grey. The lateral DMs are outlined by a dashed white line. Bar: 50 μm. CV, cardinal vein; DM, dermomyotome; M, myotome; Scl, sclerotome; SM, smooth muscle; SMA, smooth muscle actin.

Format: JPEG Size: 760KB Download file

Applebaum et al. BMC Biology 2014 12:53   doi:10.1186/s12915-014-0053-9