Additional file 1: Figure S1..
Inhibition of Notch signaling induces myogenesis. (A) HEK293 cells were co-transfected with a Dll1-encoding plasmid, with the Hes1 reporter to assay for Notch activity and with a RFP plasmid to assess transfection efficiency. Results represent the mean ± SEM of triplicate cultures (see Methods). Dll1 significantly reduced Hes1 promoter activity (*P <0.05). (B-C) Confocal images of somites electroporated with Dll1 (red) and with pTP-1Venus, a CSL-dependent reporter that reflects Notch signaling activity (green). Cells expressing Dll1 do not activate this Notch reporter cell autonomously, but rather activate it in adjacent cells. (D-G) Electroporation of the lateral DM with control GFP (D-D”), dnMAML1 (E), or Dll1 (F). (D) At 40 hours post-electroporation, cells transfected with control GFP were found in myotome (M), sclerotome (Scl) and as SM within blood vessel walls (arrows and higher magnification in D’ and D”, N = 6). (E,F) dnMAML1 and Dll1 abrogate cell migration through the sclerotome and subsequent vascular development while promoting the myotomal lineage (N = 4 and 4, respectively). Lateral DM is outlined by dotted lines. (G) Quantification (mean ± SEM). *P ≤0.05. Bar: (D-F) 50 μm; (D’, D”) 35 μm. CV, cardinal vein; DM, dermomyotome; meso, mesonephros; SEM, standard error of the mean.
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Applebaum et al. BMC Biology 2014 12:53 doi:10.1186/s12915-014-0053-9