Open Access Research article

Comparative analysis of Klebsiella pneumoniae genomes identifies a phospholipase D family protein as a novel virulence factor

Letícia MS Lery1102*, Lionel Frangeul3, Anna Tomas45, Virginie Passet67, Ana S Almeida1112, Suzanne Bialek-Davenet67, Valérie Barbe8, José A Bengoechea1245, Philippe Sansonetti129, Sylvain Brisse67 and Régis Tournebize1132*

Author Affiliations

1 Institut Pasteur - Pathogénie Microbienne Moléculaire, Paris, France

2 INSERM U786, Paris, France

3 Institut Pasteur – Genome Bioinformatics Platform, Paris, France

4 Laboratory Microbial Pathogenesis, Fundació d’Investigació Sanitària de les Illes Balears (FISIB), Bunyola, Spain

5 Program Host-Pathogen Interactions, Centro de Investigación Biomédica en Red Enfermedades Respiratorias (CibeRes), Bunyola, Spain

6 Institut Pasteur - Microbial Evolutionary Genomics, Paris, France

7 CNRS, UMR3525, Paris, France

8 CEA/IG/Genoscope, Finishing Laboratory, Evry, France

9 Microbiologie et Maladies Infectieuses, Collège de France, Paris, France

10 Present address: Biological Physics Laboratory, Institute of Biophysics Carlos Chagas Filho at the Federal University of Rio de Janeiro, Av. Carlos Chagas Filho, 373, Prédio do CCS, Bloco G, Sala G1-043, Rio de Janeiro, RJ 21941-902, Brazil

11 Present address: Cold Spring Harbor Laboratory, Bungtown Road, Cold Spring Harbor, NY 11724, USA

12 Present address: Centre for Infection and Immunity, Queen’s University Belfast, Belfast, UK

13 Present address: Plate-Forme d’Imagerie Dynamique, Imagopole, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris, Cedex 15, France

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BMC Biology 2014, 12:41  doi:10.1186/1741-7007-12-41

Published: 29 May 2014

Additional files

Additional file 1:

1) Distribution of K. pneumoniae Kp52.145 genes, according to RAST categories. Each functional category is represented in a different color. The total number of genes per category is shown. 2) pld PCR assay. A collection of 42 virulent and non virulent clones was screened by PCR for pld gene. Strains containing pld gene are indicated by (+) and the absence of pld gene is (-). 3) TLC lipid profiles of K. pneumoniae Kp52.145 wild-type (left panel) and pld mutant strains (right panel). Black circles indicate differentially expressed lipids. 4) Bacterial competition assay. Anti-bacterial activity was measured as the number of E. coli cells recovered after the co-culture with K. pneumoniae Kp52.145 wild-type and pld mutant strains. S. marcesens was used as a positive control strain. 5) List of primers used for RT-PCR analysis.

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