Additional file 2: Figure S1.
Ubiquitinated forms of VAPB are not detectable in Flag-VAPB immunoprecipitates. U2OS cells expressing Flag-VAPB from a tetracycline-inducible promoter were induced by addition of 100 ng/ml tetracycline. Flag-VAPB was immunoprecipitated after treatment with 10 μM MG132 for 1 or 2 hr, or 5 μM for 6 hr or from untreated cells (0 hr). Two exposures of Flag-VAPB immunoblots are shown. No ubiquitinated forms of Flag-VAPB can be detected with or without proteasome inhibition, not even after a very long exposure.
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Baron et al. BMC Biology 2014 12:39 doi:10.1186/1741-7007-12-39