Additional file 2: Figure S2.
FGF2 induces proliferation of the RPE. (A-H) Retinectomies were performed on E4 (Stage 24) chick eyes in the absence (A-D) or presence (E-H) of FGF2. Immunostaining using an antibody to detect BrdU (A,C,E,G), shows BrdU + RPE cells only at 24 h post-retinectomy (PR) in the presence of FGF2. Immunostaining to detect p27kip1 (B,D,F,H) is negative in the RPE only at 24 h PR in the presence of FGF2. L, lens; M, mesenchyme; RPE, retinal pigmented epithelium; *Asterisk, FGF soaked heparin bead. The scale bar in panel H represents 50 μm and applies to all images.
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Luz-Madrigal et al. BMC Biology 2014 12:28 doi:10.1186/1741-7007-12-28