Additional file 1: Figure S1.

Pluripotency inducing factors are present in the developing chick eye at E7 and after injury. (A-E) Immunohistochemical staining using antibodies against pluripotency inducing factors c-Myc (A), Sox2 (B), Klf4 (C), Lin‒28 (D) and progenitor markers Pax6 and Chx10 (E) in the posterior region of E7 (this stage was included for comparison with the 72 h PR, as the developing retina would be equivalent to the regenerating retina 72 h PR) chick eyes. Higher magnification view of the boxed areas are shown in A’-E’; dashed lines outline the RPE. (F-J) Immunofluorescence analysis of c‒Myc (F), Sox2 (G), Klf4 (H), Lin-28 (I) and progenitor markers Pax6 and Chx10 (J) in eyes 72 h PR. (K-M) Negative controls (only secondary antibodies added) for immunofluorescence analysis on eyes 72 h PR for Sox2 and Lin-28 (K), c‒Myc and Klf4 (L) and Pax6 and Chx10 (M). L, lens; M, mesenchyme; R, retina; RPE, retinal pigmented epithelium; Asterisk, FGF2-soaked heparin bead. The scale bar in panel E represents 50 μm and applies to panels A-E. Scale bar in panel E’ represents 10 μm and applies to panels A’-E’. The scale bar in panel J represents 50 μm and applies to panels F-J. The scale bar in panel M represents 50 μm and applies to panels K-M.

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Luz-Madrigal et al. BMC Biology 2014 12:28   doi:10.1186/1741-7007-12-28