Additional file 1: Figure S1.

Stages of development during metamorphosis in A. queenslandica. A: Free-swimming larva stage; the (parenchymella) larva is egg-shaped and swims with the pigmented ring and associated bundles of long cilia directed posteriorly [10]. The anterior is down. In B-F, metamorphosing postlarvae are viewed from the top. B: Settlement stage. The anterior region of the larva is attached to the substrate, onto which the larva flattens. C: Mat-formation postlarval stage, cells of the metamorphosing postlarva migrate laterally on the substrate to form a mat-like structure. Note that former posterior-ring pigmentation (pi) is still visible but is disappearing. D: Chamber-formation postlarval stage, with developing canals (ca) lined by choanocyte chambers (ch) and endopinacoderm. Note the aquiferous system composed of canals lined by choanocytes and endopinacocytes becomes evident. E: Tent-pole-formation postlarval stage, the exopinacotes covering the outer surface of the metamorphosing postlarva are lifted upwards by formation of tent-pole-like structures consisting of vertically oriented clusters of spicules and associated cells. Arrowheads show the internal tent-pole-like structures, visible here as clustering of cells. F: Juvenile (rhagon) stage with an osculum (os), marking the establishment of the functional aquiferous system. Abbreviations: lc long cilia; pr pigment ring; cc cuboidal cells. Scale bar: 100 μm (A, inset in F), 1 mm (B-F).

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Nakanishi et al. BMC Biology 2014 12:26   doi:10.1186/1741-7007-12-26