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Open Access Highly Accessed Research article

Initiating a regenerative response; cellular and molecular features of wound healing in the cnidarian Nematostella vectensis

Timothy Q DuBuc12, Nikki Traylor-Knowles3 and Mark Q Martindale2*

Author Affiliations

1 University of Hawaii, Kewalo Marine Laboratory, 41 Ahui Street, Honolulu, HI 96813, Hawaii

2 University of Florida, Whitney Marine Laboratory, 9505 Oceanshore Boulevard, St. Augustine, FL 32080, USA

3 Stanford University, Hopkins Marine Station, 120 Oceanview Blvd., Pacific Grove, CA 93950, USA

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BMC Biology 2014, 12:24  doi:10.1186/1741-7007-12-24

Published: 26 March 2014

Abstract

Background

Wound healing is the first stage of a series of cellular events that are necessary to initiate a regenerative response. Defective wound healing can block regeneration even in animals with a high regenerative capacity. Understanding how signals generated during wound healing promote regeneration of lost structures is highly important, considering that virtually all animals have the ability to heal but many lack the ability to regenerate missing structures. Cnidarians are the phylogenetic sister taxa to bilaterians and are highly regenerative animals. To gain a greater understanding of how early animals generate a regenerative response, we examined the cellular and molecular components involved during wound healing in the anthozoan cnidarian Nematostella vectensis.

Results

Pharmacological inhibition of extracellular signal-regulated kinases (ERK) signaling blocks regeneration and wound healing in Nematostella. We characterized early and late wound healing events through genome-wide microarray analysis, quantitative PCR, and in situ hybridization to identify potential wound healing targets. We identified a number of genes directly related to the wound healing response in other animals (metalloproteinases, growth factors, transcription factors) and suggest that glycoproteins (mucins and uromodulin) play a key role in early wound healing events. This study also identified a novel cnidarian-specific gene, for a thiamine biosynthesis enzyme (vitamin B synthesis), that may have been incorporated into the genome by lateral gene transfer from bacteria and now functions during wound healing. Lastly, we suggest that ERK signaling is a shared element of the early wound response for animals with a high regenerative capacity.

Conclusions

This research describes the temporal events involved during Nematostella wound healing, and provides a foundation for comparative analysis with other regenerative and non-regenerative species. We have shown that the same genes that heal puncture wounds are also activated after oral-aboral bisection, indicating a clear link with the initiation of regenerative healing. This study demonstrates the strength of using a forward approach (microarray) to characterize a developmental phenomenon (wound healing) at a phylogenetically important crossroad of animal evolution (cnidarian-bilaterian ancestor). Accumulation of data on the early wound healing events across numerous systems may provide clues as to why some animals have limited regenerative abilities.

Keywords:
Cnidarians; ERK signaling; Gastrulation; Glycoprotein; Lateral gene transfer; Metalloproteinase; Microarray; Nematostella vectensis; Regeneration; Wound healing