Additional file 9: Figure S9.

Analysis of Notch vesicle co-localization with Rab5CA and Hrs. Stage 7–8 egg chambers were dissected from hs-flp/GbeSu(H)m8-lacZ; UAS-YFP-Rab5CA/+; tub-Gal4, FRT82B, tub-Gal80/FRT82B, awdj2A4 females and stained for NICD and Hrs. Quantitative analysis of Notch vesicle co-localization with Rab5CA and Hrs was performed. In awd mutant cells 87.1% of Notch vesicles co-localizes with Rab5CA and 31.45% co-localizes with Hrs (n = 124). Co-localization was analyzed also by using ImageJ. The mean values (n = 6) of Pearson’s coefficients for NICD and Rab5CA (A) and for NICD and Hrs (B) in awd mutant cells are reported with standard deviations. (A) Co-localization image of NICD and Rab5CA and (B) co-localization image of NICD and Hrs are based on the analysis of awd mutant cells showed in Figure  8C.

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Ignesti et al. BMC Biology 2014 12:12   doi:10.1186/1741-7007-12-12