Additional file 2: Figure S2.

Presenilin expression pattern is not altered in awd mutant follicle cells. Polyclonal rabbit antibody against a C-terminal peptide in the putative hydrophilic loop region of Psn (anti-C-Psn) has been described [58]. Stage 6 and 7 egg chambers containing MARCM clones of awd mutant (marked by positive GFP expression) were stained for Psn (cyan) and NICD (red). Psn is ubiquitously expressed in intracellular punctates in both follicle cells and germ cells. No changes in either the expression level or the punctate pattern are observed in awd mutant cells. The egg chambers were dissected from hs-flp/GbeSu(H)m8-lacZ; act-Gal4, UAS-GFP/+; FRT82B, act-Gal80/FRT82B, awdJ2A4 females. Bars are 5 μm.

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Ignesti et al. BMC Biology 2014 12:12   doi:10.1186/1741-7007-12-12