Altered expression of Notch signaling target genes in awd clones. (A and C) Stage 7–8 egg chambers were dissected from hs-flp; +/+; Ubi-GFP, FRT82B/FRT82B, awdj2A4 females, and the awd mutant clones were identified by lack of GFP staining (green). (B, D-E) Stage 7 egg chambers were dissected from hs-flp/GbeSu(H)m8-lacZ; act-Gal4, UAS-GFP/+; FRT82B, act-Gal80/FRT82B, awdj2A4 females. In (A-C) the awd mutant clones were identified by lack of Awd or GFP staining (green), while in (D-E) the awd mutant clones are GFP positive. In all panels, awd mutant clones are marked with dashed lines. (A) Cyclin B (red), (B) Cut (red) and (C) Fasciclin III (FasIII; in red) are normally negatively regulated by Notch signaling, but up-regulated in awd mutant clones (lack of GFP or Awd; arrows). (D) Hindsight (Hnt; in red) is normally induced by Notch signaling, and is down-regulated in awd clones (GFP-positive; empty arrowhead). (E) The lacZ reporter gene expression driven by Notch-activated Su(H) promoter [GbeSu(H)m8-lacZ] is lost in awd clones (GFP-positive; empty arrowhead). Bars are 20 μm.
Ignesti et al. BMC Biology 2014 12:12 doi:10.1186/1741-7007-12-12