Table 1

Gene, function and number of Oct and Oct-like/nub sites in the 5′ upstream region
A. Oct sites in immune and stress response genes with ≥2 fold change in carcass or gut (a)
Gene Computed gene number Function Fold change carcass Fold change gut Oct sites Oct-like /nub
CecA1 CG1365 Antimicrobial peptide >10b >10b 3 2
CecB CG1878 Antimicrobial peptide 5.5 −1.4 1
CecC CG1373 Antimicrobial peptide 13 b.b. 2
AttC CG4740 Antimicrobial peptide 45 14 2 1
AttD CG7629 Antimicrobial peptide 8.1 −1.9 1
Def CG1385 Antimicrobial peptide 15 1.7 2
Dpt CG12763 Antimicrobial peptide >10b 4b 1
Dpt B CG10794 Antimicrobial peptide 2.7 −1.4 3
Drosocin CG10816 Antimicrobial peptide 18 4.4 1 1
Drs CG10810 Antimicrobial peptide 4.9 1.1 1
Dro2 CG32279 Antimicrobial peptide b.b 4.1 3
Dro3 CG32283 Antimicrobial peptide b.b 2.2 1
Dro4 CG32282 Antimicrobial peptide −2.5 −2.2 1
Dro5 CG10812 Antimicrobial peptide −1.2 −7.9 2 1
Listericin CG9080 Antimicrobial peptide 4.4 −1.7 2
Mtk CG8175 Antimicrobial peptide 2.5 1.5 3 1
Anp CG1361 Antimicrobial peptide b.b 154 5
LysX CG9120 Antimicrobial protein b.b −17 3
IM23 CG15066 Immune molecule 21 3.5 2 1
IM10 CG18279 Immune molecule −20 −26 2 1
PGRP-SC2 CG14745 Recognition 4.8 1.1 3
PGRP-SB1 CG9681 Recognition 4.8 1.5 1
PGRP-LD CG32912-RB/RA/RD Recognition 4.6 1.8 1
2
PGRP-LC CG4432 Recognition 3.8 2.9 2
PGRP-LB CC14704-RA/RC/RD Recognition 3.3 2.6 4
5
4 1
Tep II CG7052 Recognition 2.7 1.6 2
Tep IV CG10363 Recognition 2.4 1.8 4
GNBP3 CG5008 Recognition −2.1 −3.9 3
Sr-CIV CG3212 Scavenger receptor b.b 11 1 1
Galpha49B CG17759-RD/RE Signaling 2.5 4.2 3 1
Pirk CG15687 Signaling 2.5 4.3 5
Prx2540-2 CG11765 Peroxiredoxin 2.0 −1.4 1
Tsf1 CG6186 Iron sequestration 2.9 2.1 2
TotX CG31193 Stress peptide 3.3 2.9 2
TotM CG14027 Stress peptide 2.1 2.2 3
B. Oct sites in immune genes and in house-keeping genes with no significant fold change in carcass or gut (a)
Dro 6 CG32268 Antimicrobial peptide 1.2 1.0
Drs-like CG32274 Antimicrobial peptide 1.2 1.1
Bsk CG5680 JNK Signaling 1.1 −1.3
Gapdh1 CG12055 Glycolys 1.2 1.1
Rpl32/rp49 CG7939 Ribosomal protein −1.1 −1.1 1
Act5C CG4027 Actin 1.1 1.2
Aats-arg CG9020 Arginine tRNA syntase 1.0 −1.1

Consensus sequence of Oct sites: AT(C,G)(C,G,T)AAA(A,T) and of the Oct-like/nub site: ATTCAAAT. Fold change indicates mRNA expression levels in gut and carcass, comparing wild type versus nub1 mutant, data taken from the microarray data in Additional files 5 and 8 unless other wise indicated. Non-reliable values due to concentrations below background have been removed. A longer list of analyzed genes including the positions of the Oct sites is presented as Additional file 11. aLocation of sites within 2000 bp from the transcription start site of respective gene or up to the nearest exone of an adjacent gene. bData taken from RT-qPCR results (Figures 1 and 3). b.b., concentrations below background.

Dantoft et al.

Dantoft et al. BMC Biology 2013 11:99   doi:10.1186/1741-7007-11-99

Open Data