Expression of Nub-PD from a transgene restores normal regulation of antimicrobial peptide genes in a nub1 mutant background. Quantification of CecA1, Dipt, CecC, AttC and nub-RD mRNA by RT-qPCR in extracts of uninfected whole flies. Expression was measured in wild type (OrR; black bars), nub1 flies (nub1/nub1; UAS-nub-RD/+; dark gray bars) and nub1 flies expressing a nub-RD transgene (nub1/nub1;UAS-nub-RD/hs-Gal4; light gray bars). The data are mean values; n = 3. Statistical significance was calculated using paired t-test, *P <0.05, **P <0.01.
Dantoft et al. BMC Biology 2013 11:99 doi:10.1186/1741-7007-11-99