Research article
The Oct1 homolog Nubbin is a repressor of NF-κB-dependent immune gene expression that increases the tolerance to gut microbiota
1 Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, SE-106 91, Stockholm, Sweden
2 Huddinge Genomics Core Facilities, Department of Biosciences and Nutrition, Karolinska Institute, SE-141 87, Huddinge, Sweden
3 Present address: Laboratories for Chemical Biology Umeå (LCBU), Umeå University, SE-901 87, Umeå, Sweden
4 Present address: Department of Pathology and Cell Biology, Columbia University, New York, USA
BMC Biology 2013, 11:99 doi:10.1186/1741-7007-11-99
Published: 6 September 2013Additional files
Additional file 1:
The nub gene organization.
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Additional file 2:
Nub antibody specificity.
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Additional file 3:
Expression of CecA1, Dipt and Drs mRNA is not significantly different in OrR and nub1 flies in response to bacterial infection.
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Additional file 4:
Gene set enrichment analysis of the 642 differentially expressed transcripts in nub1 carcass.
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Additional file 5:
Data set of gene set enrichment analysis for nub1 versus wild type comparison (carcass).
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Additional file 6:
Gene set enrichment analysis of the 961 differentially expressed transcripts in nub1 gut.
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Additional file 7:
List of ‘Response process’ genes up-or down-regulated in nub1 mutants.
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Additional file 8:
Gene set enrichment analysis for nub1 versus wild type comparison (gut).
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Additional file 9:
Hierarchical clustering of ‘Immune system and Response processes’ genes.
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Additional file 10:
Hierarchical clustering of ‘Development and Differentiation’ and of ‘Nervous System’ genes.
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Additional file 11:
Presence of Oct and Oct-like sites in a selection of nub1- differentially expressed genes.
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Additional file 12:
Primer/probes sequences and details of PCR protocols.
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