Open Access Research article

The Oct1 homolog Nubbin is a repressor of NF-κB-dependent immune gene expression that increases the tolerance to gut microbiota

Widad Dantoft1, Monica M Davis1, Jessica M Lindvall2, Xiongzhuo Tang1, Hanna Uvell13, Anna Junell1, Anne Beskow14 and Ylva Engström1*

Author Affiliations

1 Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, SE-106 91, Stockholm, Sweden

2 Huddinge Genomics Core Facilities, Department of Biosciences and Nutrition, Karolinska Institute, SE-141 87, Huddinge, Sweden

3 Present address: Laboratories for Chemical Biology Umeå (LCBU), Umeå University, SE-901 87, Umeå, Sweden

4 Present address: Department of Pathology and Cell Biology, Columbia University, New York, USA

For all author emails, please log on.

BMC Biology 2013, 11:99  doi:10.1186/1741-7007-11-99

Published: 6 September 2013

Additional files

Additional file 1:

The nub gene organization.

Format: PDF Size: 91KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 2:

Nub antibody specificity.

Format: PDF Size: 1.8MB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 3:

Expression of CecA1, Dipt and Drs mRNA is not significantly different in OrR and nub1 flies in response to bacterial infection.

Format: PDF Size: 99KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 4:

Gene set enrichment analysis of the 642 differentially expressed transcripts in nub1 carcass.

Format: PDF Size: 155KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 5:

Data set of gene set enrichment analysis for nub1 versus wild type comparison (carcass).

Format: XLSX Size: 961KB Download file

Open Data

Additional file 6:

Gene set enrichment analysis of the 961 differentially expressed transcripts in nub1 gut.

Format: PDF Size: 182KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 7:

List of ‘Response process’ genes up-or down-regulated in nub1 mutants.

Format: PDF Size: 51KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 8:

Gene set enrichment analysis for nub1 versus wild type comparison (gut).

Format: XLSX Size: 1.5MB Download file

Open Data

Additional file 9:

Hierarchical clustering of ‘Immune system and Response processes’ genes.

Format: PDF Size: 6.9MB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 10:

Hierarchical clustering of ‘Development and Differentiation’ and of ‘Nervous System’ genes.

Format: PDF Size: 5.2MB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 11:

Presence of Oct and Oct-like sites in a selection of nub1- differentially expressed genes.

Format: PDF Size: 80KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 12:

Primer/probes sequences and details of PCR protocols.

Format: PDF Size: 81KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data