Table 2

Analysis of phenotypic variation between strains (ANOVA) and between sites
Phenotype Populations per genotype. Na Mean (SD) ANOVA (strain) Mean (SD) t-test
Orsay Santeuil
Population size
  Escherichia coli 6 3.136 ± 695 0.3602 3278 ± 670 2988 ± 707 0.19
  DSM 6 3.385 ± 750 4.83 × 10-4b 3369 ± 702 3402 ± 815 0.89
  BT247 6 44 ± 39 <2.2 × 10-16b 53 ± 49 34 ± 21 0.12c
Development time, days 2 to 5 1.79 ± 0.07 4.16 × 10-4b 1.79 ± 0.07 1.79 ± 0.07 0.74
Generation time, days 2 to 5 1.98 ± 0.08 3.13 × 10-6b 1.98 ± 0.077 1.97 ± 0.77 0.019b
Embryogenesis, hours 2 to 5 4.60± 0.87 NA 4.35 ± 0.70 4.85 ± 0.97 0.031b
Length, μm 2 to 6 1.089 ± 58 4.83 × 10-5b 1107 ± 33 1070 ± 72 0.023b
Width, μm 2 to 6 43.96 ± 2.63 1.33 × 10-9b 44.45 ± 1.99 43.46 ± 3.13 0.35
Volume, nl 2 to 6 1.67 ± 0.24 1.12 × 10-6b 1.73 ± 0.19 1.61 ± 0.28 0.99
Length/width ratio 2 to 6 24.79 ± 0.82 1.19 × 10-6b 24.94 ± 0.79 24.64 ± 0.83 1.40 × 10-5b

Abbreviations: BT247 nematocidal Bacillus thuringiensis NRRL B-18247, DSM non-nematocidal Bacillus thuringiensis DSM-350, NA not applicable.

aDescribes the number of replicate populations of worms (each population more than 100 worms) which were tested per genotype, thus the number of such replicate populations (N) per isolation site is therefore 20 times higher than the replicate populations per genotype (as we have 20 genotypes per isolation site).

bSignificant.

cWhen all observations per genotype were used instead of the mean per genotype, the populations from the different isolations sites were significantly different (P<0.0014).

Volkers et al.

Volkers et al. BMC Biology 2013 11:93   doi:10.1186/1741-7007-11-93

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