Open Access Research article

Transcription of a protein-coding gene on B chromosomes of the Siberian roe deer (Capreolus pygargus)

Vladimir A Trifonov1*, Polina V Dementyeva1, Denis M Larkin2, Patricia CM O’Brien3, Polina L Perelman1, Fengtang Yang4, Malcolm A Ferguson-Smith3 and Alexander S Graphodatsky1

  • * Corresponding author: Vladimir A Trifonov vlad@mcb.nsc.ru

  • † Equal contributors

Author Affiliations

1 Institute of Molecular and Cellular Biology SВ RAS, Novosibirsk, Russia

2 Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, UK

3 Cambridge Centre for Comparative Genomics, Department of Veterinary Medicine, University of Cambridge, Cambridge, UK

4 Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, UK

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BMC Biology 2013, 11:90  doi:10.1186/1741-7007-11-90

Published: 6 August 2013

Additional files

Additional file 1:

Nucleotide and amino acid sequence of Siberian roe deer (Capreolus pygargus (CPY)) CPY_a FPGT three exons, CCA (from Capreolus capreolus) and CPY_d TNNI3K first exon and nucleotide sequence of c6h cDNA.

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Additional file 2: Table S1:

The results of bovine bacterial artificial chromosome (BAC) clone mapping on chromosomes of the Siberian roe deer.

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Additional file 3: Figure S1:

Fragments of Siberian roe deer (Capreolus pygargus (CPY)) CPY1 used for quantitative polymerase chain reaction (qPCR). BTA3 is the segment of cattle chromosome 3 from the Btau_4.6.1.

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Additional file 4: Figure S2:

Flow-karyotype of the Siberian roe deer (Capreolus pygargus). ‘B’ indicates a peak containing B chromosomes.

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Additional file 5: Table S2:

Primers used for polymerase chain reaction (PCR) mapping of the Siberian roe deer (Capreolus pygargus (CPY)) CPY1 region on B chromosomes. ‘В’: presence (+) or absence (−) of the PCR product using the flow-sorted Siberian roe deer B chromosome-specific library. ‘СРY_d’: presence of the PCR product using the Siberian roe deer genomic DNA (CPY_d) genomic DNA. ‘ВТА’: presence of the PCR product using the bovine genomic DNA.

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Additional file 6: Table S3:

Primers used in quantitative real-time polymerase chain reaction (PCR).

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Additional file 7: Table S4:

Primers used for sequencing of FPGT, TNNI3K and LRRIQ3 gene fragments.

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