Additional file 6: Movie 4.

Live cell imaging of an uninfected MDM nucleofected with PH-GFP. MDMs were nucleofected with PH-GFP and imaged after 24 hours using an UltraVIEW Vox spinning disc confocal system fitted on a Nikon ECLIPSE Ti microscope equipped with a temperature and CO₂-controllable environment chamber. The movie was assembled from images taken every 10 seconds. Cell as shown in Figure 3A.

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Mlcochova et al. BMC Biology 2013 11:89   doi:10.1186/1741-7007-11-89